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Surveillance of Human Rotavirus in Wuhan, China (2011-2019): Predominance of G9P[8] and Emergence of G12.

ABSTRACT: Rotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. To learn the shift of genotypes and genetic characteristics of Rotavirus A (RVA) causing diarrhea in children and adults, a hospital-based surveillance of rotavirus was conducted in Wuhan, China from June 2011 through May 2019, and representative virus strains were phylogenetically analyzed. Among a total of 6733 stool specimens collected from both children and adults with acute gastroenteritis, RVA was detected in 25.5% (1125/4409) and 12.3% (285/2324) of specimens, respectively. G9P[⁸] was the most common genotype (74.5%), followed by G1P[⁸] (8.7%), G2P[⁴] (8.4%), and G3P[⁸] (7.3%), with G9P[⁸] increasing rapidly during the study period. The predominant genotype shifted from G1P[⁸] to G9P[⁸] in 2012-2013 epidemic season. G12P[⁶] strain RVA/Human-wt/CHN/Z2761/2019/G12P[⁶] was detected in April 2019 and assigned to G12-P[⁶]-I1-R1-C1-M1-A1-N1-T2-E1-H1 genotypes. Phylogenetic analysis revealed that VP7, VP4, VP6, VP3, NSP1, NSP2, and NSP5 genes of Z2761 clustered closely with those of Korean G12P[⁶] strain CAU_214, showing high nucleotide identities (98.0-98.8%). The NSP3 gene of Z2761 was closely related to those of G2P[⁴] and G12P[⁶] rotaviruses in Asia. All the eleven gene segments of Z2761 kept distance from those of cocirculating G9P[⁸], G1P[⁸], and G3P[⁸] strains detected in Wuhan during this study period. This is the first identification of G12 rotavirus in China. It is deduced that Z2761 is a reassortant having DS-1-like NSP3 gene in the background of G12P[⁶] rotavirus genetically close to CAU_214.

SUBMITTER: Zhou X

PROVIDER: S-EPMC7600066 | biostudies-literature |

REPOSITORIES: biostudies-literature

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