Dataset Information

Enhancement of ?-Mangostin Wound Healing Ability by Complexation with 2-Hydroxypropyl-?-Cyclodextrin in Hydrogel Formulation.

ABSTRACT: ?-Mangostin (?-M), one of the active compounds in Garcinia mangostana peel, has been effectively used in wound healing. However, its poor solubility in aqueous solution causes low bioavailability for skin ulcers, hindering its application in wound healing. The aim of this study was to improve the solubility of ?-M through complex formation with 2-hydroxypropyl-?-cyclodextrin (?-M/HP-?-CD CX) and to evaluate the healing activity of the complex. The ?-M/HP-?-CD CX was incorporated in a sodium carboxymethylcellulose hydrogel (?-M/HP-?-CD CX HG), and the in vivo healing activity was examined in mice. Evaluation of ?-M/HP-?-CD CX HG, including organoleptic evaluation, homogeneity, pH, spreadability, swelling ratio, consistency, scanning electron microscopy (SEM), and in vitro drug release, was carried out. The complex formation of ?-M/HP-?-CD CX was confirmed by FTIR and PXRD analysis. The solubility of the ?-M/HP-?-CD CX in water linearly increased about 11.7-fold compared to ?-M alone, and by 3.5-fold compared to the ?-M/HP-?-CD physical mixture (?-M/HP-?-CD CX PM). The ?-M/HP-?-CD CX HG was homogenous, the pH was found to be in the neutral range, the spread area was 5 cm, and the consistency was stable until 14 days. SEM analysis showed that ?-M/HP-?-CD CX HG surged due to the porous structure of the HG. In addition, in vitro release of ?-M from ?-M/HP-?-CD CX HG was considerably increased compared to ?-M/HP-?-CD PM HG and ?-M HG. Notably, in vivo evaluation in mice showed that ?-M/HP-?-CD CX HG significantly accelerated the wound healing ability compared to other HGs. Thus, ?-M/HP-?-CD CX HG has potential as a new formulation of ?-M for wound healing therapy.

SUBMITTER: Wathoni N

PROVIDER: S-EPMC7600479 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Publications

Enhancement of α-Mangostin Wound Healing Ability by Complexation with 2-Hydroxypropyl-β-Cyclodextrin in Hydrogel Formulation.

Wathoni Nasrul N Sari Diah Permata DP Suharyani Ine I Motoyama Keiichi K Mohammed Ahmed Fouad Abdelwahab AFA Cahyanto Arief A Abdassah Marline M Muchtaridi Muchtaridi M

Pharmaceuticals (Basel, Switzerland) 20201002 10

α-Mangostin (α-M), one of the active compounds in <i>Garcinia mangostana</i> peel, has been effectively used in wound healing. However, its poor solubility in aqueous solution causes low bioavailability for skin ulcers, hindering its application in wound healing. The aim of this study was to improve the solubility of α-M through complex formation with 2-hydroxypropyl-β-cyclodextrin (α-M/HP-β-CD CX) and to evaluate the healing activity of the complex. The α-M/HP-β-CD CX was incorporated in a sodi ...[more]

PMID: 33023196

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Project description:Development of natural protein-based fibrous scaffolds with tunable physical properties and biocompatibility is highly desirable to construct three-dimensional (3D), fully cellularized scaffolds for wound healing. Herein, we demonstrated a simple and effective technique to construct electrospun 3D fibrous scaffolds for accelerated wound healing using a photocrosslinkable hydrogel based on gelatin methacryloyl (GelMA). We found that the physical properties of the photocrosslinkable hydrogel including water retention, stiffness, strength, elasticity and degradation can be tailored by changing the light exposure time. We further observed that the optimized hydrogel fibrous scaffolds which were soft and elastic could support cell adhesion, proliferation and migration into the whole scaffolds, facilitating regeneration and formation of cutaneous tissues within two weeks. Such tunable characteristics of the fibrous GelMA scaffolds distinguished them from other reported substrates developed for reconstruction of wound defects including glutaraldehyde-crosslinked gelatin or poly (lactic-co-glycolic acid) (PLGA), whose physical and chemical properties were difficult to modify to allow cell infiltration into the 3D scaffolds for tissue regeneration. We anticipate that the ability to become fully cellularized will make the engineered GelMA fibrous scaffolds suitable for widespread applications as skin substitutes or wound dressings.Statement of significanceIn present study, we generate three-dimensional photocrosslinkable gelatin (GelMA)-based fibrous scaffolds with tunable physical and biological properties by using a combined photocrosslinking/electrospinning approach. The developed GelMA fibrous scaffolds can not only support cell viability and cell adhesion, but also facilitate cell migration and proliferation, accelerating regeneration and formation of cutaneous tissues. In addition, the physical properties of the engineered fibrous GelMA hydrogel including water retention capability, mechanical properties and biodegradability can be tuned to accommodate different patients' needs, making it a promising candidate for skin tissue engineering.

Dataset Information

Enhancement of ?-Mangostin Wound Healing Ability by Complexation with 2-Hydroxypropyl-?-Cyclodextrin in Hydrogel Formulation.

Publications

Enhancement of α-Mangostin Wound Healing Ability by Complexation with 2-Hydroxypropyl-β-Cyclodextrin in Hydrogel Formulation.

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