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Beet Pulp: An Alternative to Improve the Gut Health of Growing Pigs.

ABSTRACT: The present study aimed to investigate the effects of dietary fiber on the gut health of growing pigs. In total, 30 growing pigs with an initial average body weight of 45.8 ± 2.78 kg were divided into three groups with 10 replicates per treatment, and one pig per replicate. The treatments included a corn-soybean meal-based diet (control group, 1.5% crude fiber (CF)), corn-soybean meal + beet pulp-based diet (beet pulp group, 5.74% CF) and corn-soybean meal-based diet (feed intake-pairing group (pairing group); the feed intake was equal to the beet pulp group, 1.5% CF). The whole trial lasted 28 days. The beet pulp group had a longer length of the large intestine, higher weight of the small intestine and whole intestine, greater density of the large intestine and whole intestine, and higher villus height in the jejunum and ileum than the control group (p < 0.05). The messenger RNA (mRNA) expression levels of epidermal growth factor (EGF), glucagon-like peptide 2 (GLP-2), and glucagon-like peptide 2 receptor (GLP-2R) in the duodenum, EGF and GLP-2 in the jejunum, EGF in the ileum, and GLP-2 in the colon were higher in the beet pulp group than in the control group (p < 0.05). Moreover, the apparent total tract digestibility of crude ash, energy, dry matter (DM), and crude protein (CP) was lower in the beet pulp group than in the control group (p < 0.05), while the apparent total tract digestibility of CF, the activity of jejunal lactase, and the mRNA abundance of duodenal GLP-2 were higher in the beet pulp group than in the control and pairing groups (p < 0.05). In addition, the beet pulp group had more goblet cells in the colon, more Bifidobacterium spp. in the cecal digesta, higher concentrations of acetic acid and butyric acid in the cecal digesta, and higher mRNA abundance of duodenal regeneration protein ?? (REG-??), jejunal mucin 2 (MUC-2), and ileal G protein-coupled receptor 43 (GPR-43) than the control group (p < 0.05). However, these parameters did not differ between the control and pairing groups (p > 0.05). These findings indicate feeding a high-fiber diet (5.74% CF, obtained from beet pulp) to pigs could modulate the gut microbiota composition, increase the short-chain fatty-acid (SCFA) content in the hindgut, and improve gut health, which is independent of the feed intake.

SUBMITTER: Diao H

PROVIDER: S-EPMC7600662 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Beet Pulp: An Alternative to Improve the Gut Health of Growing Pigs.

Diao Hui H Jiao Anran A Yu Bing B He Jun J Zheng Ping P Yu Jie J Luo Yuheng Y Luo Junqiu J Mao Xiangbing X Chen Daiwen D

Animals : an open access journal from MDPI 20201013 10

The present study aimed to investigate the effects of dietary fiber on the gut health of growing pigs. In total, 30 growing pigs with an initial average body weight of 45.8 ± 2.78 kg were divided into three groups with 10 replicates per treatment, and one pig per replicate. The treatments included a corn-soybean meal-based diet (control group, 1.5% crude fiber (CF)), corn-soybean meal + beet pulp-based diet (beet pulp group, 5.74% CF) and corn-soybean meal-based diet (feed intake-pairing group ( ...[more]

PMID: 33065992

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Project description:In 2006, the European Union (EU) has decided to forbid use of antibiotics as growth promoters. Although many researches had been conducted about fiber source as alternatives of antibiotics, there are still lack of reports in the literature about the optimum level of sugar beet pulp supplementation, affecting growth performance and nutrient digestibility in weaning pigs. Therefore, different level of sugar beet pulp was added to diets to determine the effects of sugar beet pulp supplementation on growth performance, nutrient digestibility, fecal microflora, blood profile and incidence of diarrhea in weaning pigs.A total of 200 weaning pigs [(Yorkshire × Landrace) × Duroc], averaging 9.01 ± 1.389 kg of initial body weight were, allotted to 5 treatments in a randomized complete block (RCB) design. Each treatment was composed of 4 replicates with 10 pigs per pen. The treatments were control treatment: Corn-SBM basal diet + ZnO (phase 1: 0.05%; phase 2; 0.03%) and four different levels of sugar beet pulp were supplemented in Corn-SBM basal diet (3, 6, 9 or 12%). Two phase feeding programs (phase 1: 1-2 weeks; phase 2: 3-5 weeks) were used for 5 week of growth trial.In feeding trial, there were no significant differences in growth performance and incidence of diarrhea among treatments. The E.coli counts were not significantly different among dietary treatments but linear response was observed in Lactobacillus counts as sugar beet pulp supplementation increased (P < 0.05). In addition, IGF-1, IgA and IgG were not affected by dietary treatments. However, the BUN concentration was decreased when pigs were fed the treatments of diets with SBP compared to that of control treatment (P < 0.05). In nutrient digestibility, crude fiber and NDF digestibilities were improved as the sugar beet pulp increased (P < 0.05). However, digestibilities of crude ash, crude fat, crude fiber and nitrogen retention were not affected by dietary sugar beet pulp levels.This experiment demonstrated that sugar beet pulp can be supplemented in weaning pigs' diet instead of ZnO to prevent postweaning diarrhea without any detrimental effect on growth performance.

Project description:This study investigated the effects of xylanase supplementations with cereal-based diets on nutrient digestibility and gut microbiota of growing pigs. A total of 96 individually penned pigs (initial BW = 22.7 ± 0.65 kg) were allotted to 12 treatments and subjected to a completely randomized block design experiment. Pigs in each treatment were fed an isocaloric wheat-based or corn-based diet with or without 1 of 5 types of xylanase supplements (XA, XB, XC, XD, XE). On d 42, all piglets were euthanized to obtain ileal and cecal digesta for microbial analysis, which involved high-throughput sequencing of the V1 - V3 regions of 16S rRNA gene. Corn- and wheat-based diets differed (P < 0.05) in digestion characteristics. Dietary treatments affected the alpha- and beta-diversities of microbiota in the cecum but not in the ileum. The wheat-based diet increased (P < 0.05) alpha-diversity and clustered separately (P < 0.05) compared with the corn-based diet. Wheat-based diet also promoted the relative abundance of genus (g.) Succinivibrio while corn-based diet promoted the proportion of family (f.) Veillonellaceae in the community. Among xylanases, only XC within the wheat-based diet altered (P < 0.05) the beta-diversity of the cecal microbiota compared with control. For each cereal-based diet and compared with the controls, xylanase treatments affected (P < 0.05) the proportions of 5 bacterial taxa in the ileum (f. Peptostreptococcaceae, order [o.] Streptophyta, f. Clostridiaceae, g. Clostridium and g. Streptococcus) and 8 in the cecum (g. Lactobacillus, g. Streptococcus, class [c.] Clostridia, f. Clostridiaceae, g. Megasphaera, g. Prevotella, g. Roseburia and f. Ruminococcaceae). Network analysis showed that across diets under control treatments, Bacteroidetes was the most influential phylum promoting cooperative relationships among members of the ileum and cecum microbiota. Xylanase treatment, however, reduced the influence of Bacteroidetes and promoted a large number of hub taxa majority of which belonged to the Firmicutes phylum. To maximize the efficiency of xylanase supplementation, our data suggest that xylanase C originated from Bacillus subtilis was more effective when applied to wheat-based diets, while xylanase A originated from Fusarium verticillioides was more beneficial when applied to corn-based diets.

Dataset Information

Beet Pulp: An Alternative to Improve the Gut Health of Growing Pigs.

Publications

Beet Pulp: An Alternative to Improve the Gut Health of Growing Pigs.

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