Dataset Information

Wolffia globosa-Mankai Plant-Based Protein Contains Bioactive Vitamin B₁₂ and Is Well Absorbed in Humans.

ABSTRACT:

Background

Rare plants that contain corrinoid compounds mostly comprise cobalamin analogues, which may compete with cobalamin (vitamin B₁₂ (B₁₂)) metabolism. We examined the presence of B₁₂ in a cultivated strain of an aquatic plant: Wolffia globosa (Mankai), and predicted functional pathways using gut-bioreactor, and the effects of long-term Mankai consumption as a partial meat substitute, on serum B₁₂ concentrations.

Methods

We used microbiological assay, liquid-chromatography/electrospray-ionization-tandem-mass-spectrometry (LC-MS/MS), and anoxic bioreactors for the B₁₂ experiments. We explored the effect of a green Mediterranean/low-meat diet, containing 100 g of frozen Mankai shake/day, on serum B₁₂ levels during the 18-month DIRECT-PLUS (ID:NCT03020186) weight-loss trial, compared with control and Mediterranean diet groups.

Results

The B₁₂ content of Mankai was consistent at different seasons (p = 0.76). Several cobalamin congeners (Hydroxocobalamin(OH-B₁₂); 5-deoxyadenosylcobalamin(Ado-B₁₂); methylcobalamin(Me-B₁₂); cyanocobalamin(CN-B₁₂)) were identified in Mankai extracts, whereas no pseudo B₁₂ was detected. A higher abundance of 16S-rRNA gene amplicon sequences associated with a genome containing a KEGG ortholog involved in microbial B₁₂ metabolism were observed, compared with control bioreactors that lacked Mankai. Following the DIRECT-PLUS intervention (n = 294 participants; retention-rate = 89%; baseline B₁₂ = 420.5 ± 187.8 pg/mL), serum B₁₂ increased by 5.2% in control, 9.9% in Mediterranean, and 15.4% in Mankai-containing green Mediterranean/low-meat diets (p = 0.025 between extreme groups).

Conclusions

Mankai plant contains bioactive B₁₂ compounds and could serve as a B₁₂ plant-based food source.

SUBMITTER: Sela I

PROVIDER: S-EPMC7600829 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

<i>Wolffia globosa-Mankai</i> Plant-Based Protein Contains Bioactive Vitamin B<sub>12</sub> and Is Well Absorbed in Humans.

Sela Ilan I Yaskolka Meir Anat A Brandis Alexander A Krajmalnik-Brown Rosa R Zeibich Lydia L Chang Debbie D Dirks Blake B Tsaban Gal G Kaplan Alon A Rinott Ehud E Zelicha Hila H Arinos Shira S Ceglarek Uta U Isermann Berend B Lapidot Miri M Green Ralph R Shai Iris I

Nutrients 20201008 10

<h4>Background</h4>Rare plants that contain corrinoid compounds mostly comprise cobalamin analogues, which may compete with cobalamin (vitamin B<sub>12</sub> (B<sub>12</sub>)) metabolism. We examined the presence of B<sub>12</sub> in a cultivated strain of an aquatic plant: <i>Wolffia globosa</i> (Mankai), and predicted functional pathways using gut-bioreactor, and the effects of long-term Mankai consumption as a partial meat substitute, on serum B<sub>12</sub> concentrations.<h4>Methods</h4>We ...[more]

PMID: 33049929

Similar Datasets

Project description:BackgroundThe plant body in duckweed species has undergone reduction and simplification from the ancient Spirodela species towards more derived Wolffia species. Among the five duckweed genera, Wolffia members are rootless and represent the smallest and most reduced species. A better understanding of Wolffia frond architecture is necessary to fully explore duckweed evolution.ResultsWe conducted a comprehensive study of the morphology and anatomy of Wolffia globosa, the only Wolffia species in China. We first used X-ray microtomography imaging to reveal the three-dimensional and internal structure of the W. globosa frond. This showed that new fronds rapidly budded from the hollow reproductive pocket of the mother fronds and that several generations at various developmental stages could coexist in a single W. globosa frond. Using light microscopy, we observed that the meristem area of the W. globosa frond was located at the base of the reproductive pocket and composed of undifferentiated cells that continued to produce new buds. A single epidermal layer surrounded the W. globosa frond, and the mesophyll cells varied from small and dense palisade-like parenchyma cells to large, vacuolated cells from the ventral to the dorsal part. Furthermore, W. globosa fronds contained all the same organelles as other angiosperms; the most prominent organelles were chloroplasts with abundant starch grains.ConclusionsOur study revealed that the reproductive strategy of W. globosa plants enables the rapid accumulation of biomass and the wide distribution of this species in various habitats. The reduced body plan and size of Wolffia are consistent with our observation that relatively few cell types are present in these plants. We also propose that W. globosa plants are not only suitable for the study of structural reduction in higher plants, but also an ideal system to explore fundamental developmental processes of higher plants that cannot be addressed using other model plants.

Project description:Space agriculture, pivotal for sustainable extraterrestrial missions, requires plants that can adapt to altered gravitational conditions. This study delves into the adaptive responses to altered gravity of Wolffia globosa, an aquatic plant known for its rapid growth and high nutritional value. The research aimed to analyse the effect of simulated microgravity and hypergravity on relative growth rate (RGR), morphological characteristics, protein content, and the correlation between plant size and growth rate of Wolffia globosa. The study highlighted the responses of the species to altered gravity, uncovering inherent variability among seven different clones of W. globosa. Results show a base variability among clones in terms of RGR, size and protein content. Furthermore, some clones are affected by simulated microgravity, showing a decrease in RGR. Differently, under hypergravity, clones showed RGR higher than in 1 g control, therefore revealing a novel plant response to hypergravity. Morphological adaptations to gravity alterations were also evident. Among the studied clones, significant morphological changes were observed, further underlining the peculiar adaptation to the hypergravity environment. Differently, under simulated microgravity, morphology was generally stable across clones. A key finding of the study was the significant negative correlation between RGR and the physical dimensions of the plants: the fastest growth was associated with the smallest dimensions of the plants. This correlation might have practical implications in selecting clones for space cultivation, that leads to compact yet highly productive clones. The analysis of the protein content of all the clones revealed mostly no significant changes under hypergravity. Otherwise, a general decrease in protein content was observed under simulated microgravity. Overall, the study confirms the suitability of W. globosa for space agriculture and provides new insights into the perspective of using W. globosa as an alternative crop species for protein production for manned Space missions. Furthermore, it underscores the need for focusing on the clones and the selection of the W. globosa plants that are best adapted to the environmental conditions of space; therefore, selecting those with the best combination of biomass production (by means of growth rate, size), and protein content.

Dataset Information

Wolffia globosa-Mankai Plant-Based Protein Contains Bioactive Vitamin B₁₂ and Is Well Absorbed in Humans.

Background

Methods

Results

Conclusions

Publications

<i>Wolffia globosa-Mankai</i> Plant-Based Protein Contains Bioactive Vitamin B<sub>12</sub> and Is Well Absorbed in Humans.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Wolffia globosa-Mankai Plant-Based Protein Contains Bioactive Vitamin B12 and Is Well Absorbed in Humans.

Background

Methods

Results

Conclusions

Publications

<i>Wolffia globosa-Mankai</i> Plant-Based Protein Contains Bioactive Vitamin B<sub>12</sub> and Is Well Absorbed in Humans.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Wolffia globosa-Mankai Plant-Based Protein Contains Bioactive Vitamin B₁₂ and Is Well Absorbed in Humans.