Targeting Nanodiamonds to the Nucleus in Yeast Cells.
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ABSTRACT:
SUBMITTER: Morita A
PROVIDER: S-EPMC7601435 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
Targeting Nanodiamonds to the Nucleus in Yeast Cells.
Morita Aryan A Hamoh Thamir T Sigaeva Alina A Norouzi Neda N Nagl Andreas A van der Laan Kiran J KJ Evans Emily P P EPP Schirhagl Romana R
Nanomaterials (Basel, Switzerland) 20201002 10
Nanodiamonds are widely used for drug delivery, labelling or nanoscale sensing. For all these applications it is highly beneficial to have control over the intracellular location of the particles. For the first time, we have achieved targeting the nucleus of yeast cells. In terms of particle uptake, these cells are challenging due to their rigid cell wall. Thus, we used a spheroplasting protocol to remove the cell wall prior to uptake. To achieve nuclear targeting we used nanodiamonds, which wer ...[more]
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