Unknown

Dataset Information

0

FGF2-Derived PeptibodyF2-MMAE Conjugate for Targeted Delivery of Cytotoxic Drugs into Cancer Cells Overexpressing FGFR1.


ABSTRACT: Fibroblast growth factor receptors (FGFRs) are emerging targets for directed cancer therapy. Presented here is a new FGFR1-targeting conjugate, the peptibodyF2, which employs peptibody, a fusion of peptide and the Fc fragment of human IgG as a selective targeting agent and drug carrier. Short peptide based on FGF2 sequence was used to construct a FGFR1-targeting peptibody. We have shown that this peptide ensures specific delivery of peptibodyF2 into FGFR1-expressing cells. In order to use peptibodyF2 as a delivery vehicle for cytotoxic drugs, we have conjugated it with MMAE, a drug widely used in antibody-drug conjugates for targeted therapy. Resulting conjugate shows high and specific cytotoxicity towards FGFR1-positive cells, i.e., squamous cell lung carcinoma NCI-H520, while remaining non-toxic for FGFR1-negative cells. Such peptibody-drug conjugate can serve as a basis for development of therapy for tumors with overexpressed or malfunctioning FGFRs.

SUBMITTER: Jendryczko K 

PROVIDER: S-EPMC7602595 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

FGF2-Derived PeptibodyF2-MMAE Conjugate for Targeted Delivery of Cytotoxic Drugs into Cancer Cells Overexpressing FGFR1.

Jendryczko Karolina K   Chudzian Julia J   Skinder Natalia N   Opaliński Łukasz Ł   Rzeszótko Jakub J   Wiedlocha Antoni A   Otlewski Jacek J   Szlachcic Anna A  

Cancers 20201015 10


Fibroblast growth factor receptors (FGFRs) are emerging targets for directed cancer therapy. Presented here is a new FGFR1-targeting conjugate, the peptibodyF2, which employs peptibody, a fusion of peptide and the Fc fragment of human IgG as a selective targeting agent and drug carrier. Short peptide based on FGF2 sequence was used to construct a FGFR1-targeting peptibody. We have shown that this peptide ensures specific delivery of peptibodyF2 into FGFR1-expressing cells. In order to use peptib  ...[more]

Similar Datasets

| S-EPMC5450166 | biostudies-literature
| S-EPMC7352346 | biostudies-literature
| S-EPMC9658017 | biostudies-literature
| S-EPMC5617978 | biostudies-other
| S-EPMC3129787 | biostudies-literature
| S-EPMC4651790 | biostudies-literature
| S-EPMC5085145 | biostudies-other
| S-EPMC6308115 | biostudies-literature
| S-EPMC6363389 | biostudies-literature
| S-EPMC7140114 | biostudies-literature