Unknown

Dataset Information

0

Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability.


ABSTRACT: G-rich DNA repeats that can form G-quadruplex structures are prevalent in bacterial genomes and are frequently associated with regulatory regions of genes involved in virulence, antigenic variation, and antibiotic resistance. These sequences are also inherently mutagenic and can lead to changes affecting cell survival and adaptation. Transcription of the G-quadruplex-forming repeat (G3T)n in E. coli, when mRNA comprised the G-rich strand, promotes G-quadruplex formation in DNA and increases rates of deletion of G-quadruplex-forming sequences. The genomic instability of G-quadruplex repeats may be a source of genetic variability that can influence alterations and evolution of bacteria. The DNA chaperone Hfq is involved in the genetic instability of these G-quadruplex sequences. Inactivation of the hfq gene decreases the genetic instability of G-quadruplex, demonstrating that the genomic instability of this regulatory element can be influenced by the E. coli highly pleiotropic Hfq protein, which is involved in small noncoding RNA regulation pathways, and DNA organization and packaging. We have shown previously that the protein binds to and stabilizes these sequences, increasing rates of their genomic instability. Here, we extend this analysis to characterize the role of the C-terminal domain of Hfq protein in interaction with G-quadruplex structures. This allows to better understand the function of this specific region of the Hfq protein in genomic instability.

SUBMITTER: Parekh VJ 

PROVIDER: S-EPMC7603069 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Crucial Role of the C-Terminal Domain of Hfq Protein in Genomic Instability.

Parekh Virali J VJ   Wien Frank F   Grange Wilfried W   De Long Thomas A TA   Arluison Véronique V   Sinden Richard R RR  

Microorganisms 20201017 10


G-rich DNA repeats that can form G-quadruplex structures are prevalent in bacterial genomes and are frequently associated with regulatory regions of genes involved in virulence, antigenic variation, and antibiotic resistance. These sequences are also inherently mutagenic and can lead to changes affecting cell survival and adaptation. Transcription of the G-quadruplex-forming repeat (G<sub>3</sub>T)<sub>n</sub> in <i>E. coli</i>, when mRNA comprised the G-rich strand, promotes G-quadruplex format  ...[more]

Similar Datasets

| S-EPMC4111955 | biostudies-literature
| S-EPMC6168691 | biostudies-literature
| S-EPMC8254114 | biostudies-literature
| S-EPMC3477312 | biostudies-literature
| S-EPMC3561953 | biostudies-literature
| S-EPMC7105322 | biostudies-literature
| S-EPMC7023247 | biostudies-literature
| S-EPMC6771729 | biostudies-literature
| S-EPMC4090077 | biostudies-literature
| S-EPMC6123778 | biostudies-literature