Ontology highlight
ABSTRACT:
SUBMITTER: Gao N
PROVIDER: S-EPMC7604293 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Frontiers in cell and developmental biology 20201019
Compact CRISPR/Cas9 systems that can be delivered by AAV for <i>in vivo</i> genome editing hold great promise for clinical applications. <i>Brevibacillus laterosporus</i> Cas9 (BlatCas9) is a compact Cas9 nuclease that has been identified for plant genome editing. Here, we characterize BlatCas9 as an alternative tool for mammalian genome editing. We demonstrate that BlatCas9 prefers a N4CNAA protospacer adjacent motif (PAM), but N4C PAM is also editable in mammalian cells. We next demonstrate th ...[more]