[Single nucleotide polymorphism of rs28416520 in Piwil1 gene promoter region is associated with an increased risk of gastric cancer].

ABSTRACT:

Objective

To analyze the correlation between the single nucleotide polymorphisms (SNPs) in the promoter of Piwil1 gene and gastric cancer.

Methods

The expression of Piwil1 mRNA in the tumor tissues of 3 patients with gastric cancer was detected by RT-qPCR, and RNA-Sequencing data from the Cancer RNA-Seq Nexus were analyzed for Piwil1 mRNA expression in gastric patients. Blood samples were collected from 24 gastric cancer patients and 29 healthy control subjects for PCR amplification of Piwil1 gene promoter region. The SNP loci in the promoter region of Piwil1 gene were determined by direct sequencing, and the results were analyzed by SnapGene software.

Results

Analysis of the data from Cancer RNA-Seq Nexus and the results of RT-qPCR

SUBMITTER: Zhenzhen LI 

PROVIDER: S-EPMC7606242 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature