Variations in ribosomal DNA copy numbers in a genome of Trichophyton interdigitale.

ABSTRACT:

Background

Ribosomal DNA (rDNA) reportedly has multiple copies in the fungal genome. The internal transcribed spacer (ITS) region in rDNA is useful for investigating relationships between close taxonomic relatives. Thus, ITS has been widely used as a target gene in medical mycology for the detection of pathogenic fungi and identification of fungal species. However, the rDNA copy number in a genome of Trichophyton interdigitale, the pathogen causing dermatophytosis, currently remains unknown.

Objective

Clarification of the rDNA copy number in a genome of T. interdigitale.

Methods

rDNA copy numbers in 64 clinical isolates of T. interdigitale were examined using quantitative real-time PCR (qPCR) with the absolute quantitative method targeting TruMDR2, a single-copy contr

SUBMITTER: Iwanaga T 

PROVIDER: S-EPMC7607385 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature