Dataset Information

Phylogenetical analysis of partially sequenced cytb gene of Haemoproteus columbae in pigeons and its pathological lesions in Egypt.

ABSTRACT: Background:Haemoproteus columbae is widely distributed in tropical and subtropical regions, causing pseudomalaria in pigeons. Aims:The current study aimed to characterize the phylogenetic position of H. columbae in pigeons in Sharkia province, Egypt, based on partial sequencing of the cytb gene as the conserved regions. The "DNA barcode" of the cytb gene helps in designing primers that can be used to amplify the same gene in the related haemosporidians. Methods: One hundered blood samples were collected from domestic pigeons to identify H. columbae by polymerase chain reaction (PCR) and detect its relationship with other related haemosporidians. Results:Weight losses of 60%, anemia 40%, low growth rates 26.67%, diarrhea 76.67%, dyspnea 66.67%, some neurological symptoms 33.33%, and death 16.67% were observed in the studied birds. Post-mortem examinations showed chocolate-brown appearance of the livers of the birds and congested parenchymatous organs. Microscopical examinations of Giemsa stained blood smears (n=100) revealed a 30% infection rate. The obtained infection percentages were more pronounced in males (35.71%) than females (16.66%) and more in adults (57.14%) than young pigeons (15.38%). The present sequence of H. columbae was deposited in GenBank under accession No.: MH345964 and shows 100% identity with other related Haemoproteus species in the Sao Paulo Zoo, Brazil (KU131585 and KU131583) and the UK (KX832581 and KX832586). Conclusion:This study concluded that the accurate diagnosis of H. coulmbae infection in pigeons by specific primers will help with the early treatment of affected cases, especially in the presence of the immature forms, and can thus avoid the noticed clinical signs and the induced pathological lesions mentioned in our study.

SUBMITTER: Hala MNT

PROVIDER: S-EPMC7608044 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Phylogenetical analysis of partially sequenced <i>cytb</i> gene of <i>Haemoproteus columbae</i> in pigeons and its pathological lesions in Egypt.

Hala M N T MNT Mona M I A MIA Heba M A MA

Iranian journal of veterinary research 20200101 3

<h4>Background</h4><i>Haemoproteus columbae</i> is widely distributed in tropical and subtropical regions, causing pseudomalaria in pigeons.<h4>Aims</h4>The current study aimed to characterize the phylogenetic position of <i>H. columbae</i> in pigeons in Sharkia province, Egypt, based on partial sequencing of the <i>cytb</i> gene as the conserved regions. The "DNA barcode" of the <i>cytb</i> gene helps in designing primers that can be used to amplify the same gene in the related haemosporidians. ...[more]

PMID: 33178298

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Project description:In this investigation, the incidence and intensity of gastrointestinal parasites of domestic pigeons were evaluated, additionally, in vitro and in vivo evaluation of the antiparasitic activity of chitosan nanoparticles against the most predominant gut parasite. Therefore, 240 domestic pigeons (160 adults and 80 squabs) obtained from different localities in Giza governorate, Egypt, from February to July 2021, were subjected to parasitological and postmortem examination. The results revealed that 97% of pigeons were vulnerable to single or mixed gastrointestinal parasites. The detected helminths were identified as Capillaria columbae (C. columbae) with a total incidence of (12.5%), Ascaridia columbae (A. columbae) (83.3.%), Heterakis gallinarum (H. gallinarum) (18.7%), Raillietina cesticillus (R. cesticillus) (7.5%), Raillietina echinobothrida (R. echinobothrida) (29%), Choanotaenia infundibulum (C. infundibulum) (22.9%), Davainea proglottina (D. proglottina) (26.6%), and Cotugnia proglottina (C. proglottina) (14.5%). At the same time, the identified protozoan parasites were Trichomonas gallinae (T. gallinae), and Eimeria columbae (E. columbae), with a total incidence of 25 and 79%, respectively. Helminths and Eimeria infections were higher in adults than squabs, while T. gallinae infection was reported with a higher incidence in squabs (62.5%) than adults (6.2%). From our findings, A. columbae was the most predominant gut parasite in the examined pigeons. Thus, it was subjected to in vitro and in vivo treatment with chitosan nanoparticles. Serum and tissue samples were collected from the birds which have been used in the in vitro study to evaluate the oxidative stress markers as malondialdehyde (MDA), Nitric oxide levels and Tumor Necrosis Factor-alpha (TNF-α) and Interleukin-1β activity also, chitosan nanoparticles- treated worm ultrastructure were determined using scanning electron microscopy (SEM). Finally, we recommend periodic monitoring of pigeon's farm to detect the parasitic infestation, and from our results, we recommend chitosan nanoparticles as a potent nematocidal agent.

Dataset Information

Phylogenetical analysis of partially sequenced cytb gene of Haemoproteus columbae in pigeons and its pathological lesions in Egypt.

Publications

Phylogenetical analysis of partially sequenced <i>cytb</i> gene of <i>Haemoproteus columbae</i> in pigeons and its pathological lesions in Egypt.

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