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Measuring hemoglobin spectra: searching for carbamino-hemoglobin.


ABSTRACT: The arterial carbon dioxide (CO2) partial pressure PaCO2 is a clinically relevant variable. However, its measurement requires arterial blood sampling or bulky and expensive transcutaneous PtcCO2 meters. While the spectrophotometric determination of hemoglobin species-such as oxy-hemoglobin (O2Hb) and deoxy-hemoglobin (HHb)-allowed for the development of pulse oximetry, the measurement of CO2 blood content with minimal discomfort has not been addressed yet. Characterizing human carbamino-hemoglobin (CO2Hb) absorption spectrum, which is missing from the literature. Providing the theoretical background that will allow for transcutaneous, noninvasive PaCO2 measurements. A tonometry-based approach was used to obtain gas-equilibrated, lysed, diluted human blood. Equilibration was performed with both CO2, dinitrogen (N2), and ambient air. Spectrophotometric measurements were carried out on the 235- to 1000-nm range. A theoretical background was also derived from that of pulse oximetry. The absorption spectra of both CO2Hb and HHb were extremely close and comparable with that of state-of-the-art HHb. The above-mentioned theoretical background led to an estimated relative error above 30% on the measured amount of CO2Hb in a subject's blood. Auxiliary measurements revealed that the use of ethylene diamine tetraacetic acid did not interfere with spectrophotometric measurements, whereas sodium metabisulfite did. CO2Hb absorption spectrum was measured for the first time. Such spectrum being close to that of HHb, the use of a theoretical background based on pulse oximetry theory for noninvasive PaCO2 measurement seems extremely challenging.

SUBMITTER: Dervieux E 

PROVIDER: S-EPMC7610246 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Measuring hemoglobin spectra: searching for carbamino-hemoglobin.

Dervieux Emmanuel E   Bodinier Quentin Q   Uhring Wilfried W   Théron Michaël M  

Journal of biomedical optics 20201001 10


<h4>Significance</h4>The arterial carbon dioxide (CO2) partial pressure PaCO2 is a clinically relevant variable. However, its measurement requires arterial blood sampling or bulky and expensive transcutaneous PtcCO2 meters. While the spectrophotometric determination of hemoglobin species-such as oxy-hemoglobin (O2Hb) and deoxy-hemoglobin (HHb)-allowed for the development of pulse oximetry, the measurement of CO2 blood content with minimal discomfort has not been addressed yet.<h4>Aim</h4>Charact  ...[more]

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