Ontology highlight
ABSTRACT:
SUBMITTER: Sawa-Makarska J
PROVIDER: S-EPMC7610778 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Sawa-Makarska Justyna J Baumann Verena V Coudevylle Nicolas N von Bülow Sören S Nogellova Veronika V Abert Christine C Schuschnig Martina M Graef Martin M Hummer Gerhard G Martens Sascha S
Science (New York, N.Y.) 20200901 6508
Autophagosomes form de novo in a manner that is incompletely understood. Particularly enigmatic are autophagy-related protein 9 (Atg9)-containing vesicles that are required for autophagy machinery assembly but do not supply the bulk of the autophagosomal membrane. In this study, we reconstituted autophagosome nucleation using recombinant components from yeast. We found that Atg9 proteoliposomes first recruited the phosphatidylinositol 3-phosphate kinase complex, followed by Atg21, the Atg2-Atg18 ...[more]