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Live cell tracking of macrophage efferocytosis during Drosophila embryo development in vivo.


ABSTRACT: Apoptosis of cells and their subsequent removal through efferocytosis occurs in nearly all tissues during development, homeostasis, and disease. However, it has been difficult to track cell death and subsequent corpse removal in vivo. We developed a genetically encoded fluorescent reporter, CharON (Caspase and pH Activated Reporter, Fluorescence ON), that could track emerging apoptotic cells and their efferocytic clearance by phagocytes. Using Drosophila expressing CharON, we uncovered multiple qualitative and quantitative features of coordinated clearance of apoptotic corpses during embryonic development. When confronted with high rates of emerging apoptotic corpses, the macrophages displayed heterogeneity in engulfment behaviors, leading to some efferocytic macrophages carrying high corpse burden. Overburdened macrophages were compromised in clearing wound debris. These findings reveal known and unexpected features of apoptosis and macrophage efferocytosis in vivo.

SUBMITTER: Raymond MH 

PROVIDER: S-EPMC7612538 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Live cell tracking of macrophage efferocytosis during <i>Drosophila</i> embryo development in vivo.

Raymond Michael H MH   Davidson Andrew J AJ   Shen Yi Y   Tudor Daniel R DR   Lucas Christopher D CD   Morioka Sho S   Perry Justin S A JSA   Krapivkina Julia J   Perrais David D   Schumacher Linus J LJ   Campbell Robert E RE   Wood Will W   Ravichandran Kodi S KS  

Science (New York, N.Y.) 20220310 6585


Apoptosis of cells and their subsequent removal through efferocytosis occurs in nearly all tissues during development, homeostasis, and disease. However, it has been difficult to track cell death and subsequent corpse removal in vivo. We developed a genetically encoded fluorescent reporter, CharON (Caspase and pH Activated Reporter, Fluorescence ON), that could track emerging apoptotic cells and their efferocytic clearance by phagocytes. Using <i>Drosophila</i> expressing CharON, we uncovered mu  ...[more]

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