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Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia.

ABSTRACT: BACKGROUND:Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but promising findings about its utility in diagnosing SIRS and predicting the outcome in diseased horses, were recently highlighted. The objective of the study was to investigate the behaviour of PON-1 in horses after experimentally induced endotoxemia. To this aim, PON-1 activity was measured on 66 plasma samples collected from six clinically healthy mares, previously included in another study, before and at multiple time points between 12 and 240?h after intravenous infusion of Escherichia coli O55:B5 lipopolysaccharide (LPS). RESULTS:Compared with baseline values, a progressive transient decrease of PON-1 activity was observed starting from 24?h post-infusion, with lowest values observed between 3 to 7?days post-infusion, followed by a normalisation to pre-infusion levels the tenth day. CONCLUSIONS:The results of this study suggest that measurement and monitoring of PON-1 activity might be useful to evaluate progression and recovery from endotoxemia in horses. Further studies in horses with naturally occurring sepsis are warranted.

SUBMITTER: Scavone D

PROVIDER: S-EPMC7641807 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia.

Scavone Donatella D Sgorbini Micaela M Borges Alexandre S AS Oliveira-Filho José P JP Vitale Valentina V Paltrinieri Saverio S

BMC veterinary research 20201104 1

<h4>Background</h4>Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but promising findings about its utility in diagnosing SIRS and predicting the outcome in diseased horses, were recently highlighted. The objective of the study was to investigate the behaviour of PON-1 in horses after experimentally induced endo ...[more]

PMID: 33148245

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Project description:The role of early, serial measurements of protein biomarkers in sepsis-induced acute respiratory distress syndrome (ARDS) is not clear.ObjectivesTo determine the differences in soluble receptor for advanced glycation end-products (sRAGEs), angiopoietin-2, and surfactant protein-D (SP-D) levels and their changes over time between sepsis patients with and without ARDS.Design setting and participantsProspective observational cohort study of adult patients admitted to the medical ICU at Grady Memorial Hospital within 72 hours of sepsis diagnosis.Main outcomes and measuresPlasma sRAGE, angiopoietin-2, and SP-D levels were measured for 3 consecutive days after enrollment. The primary outcome was ARDS development, and the secondary outcome of 28-day mortality. The biomarker levels and their changes over time were compared between ARDS and non-ARDS patients and between nonsurvivors and survivors.ResultsWe enrolled 111 patients, and 21 patients (18.9%) developed ARDS. The three biomarker levels were not significantly different between ARDS and non-ARDS patients on all 3 days of measurement. Nonsurvivors had higher levels of all three biomarkers than did survivors on multiple days. The changes of the biomarker levels over time were not different between the outcome groups. Logistic regression analyses showed association between day 1 SP-D level and mortality (odds ratio, 1.52; 95% CI, 1.03-2.24; p = 0.03), and generalized estimating equation analyses showed association between angiopoietin-2 levels and mortality (estimate 0.0002; se 0.0001; p = 0.04).Conclusions and relevanceAmong critically ill patients with sepsis, sRAGE, angiopoietin-2, and SP-D levels were not significantly different between ARDS and non-ARDS patients but were higher in nonsurvivors compared with survivors. The trend toward higher levels of sRAGE and SP-D, but not of angiopoietin-2, in ARDS patients may indicate the importance of epithelial injury in sepsis-induced ARDS. Changes of the biomarker levels over time were not different between the outcome groups.

Dataset Information

Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia.

Publications

Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia.

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