Ontology highlight
ABSTRACT:
SUBMITTER: Cash JN
PROVIDER: S-EPMC7642776 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Cash Jennifer N JN Kearns Sarah S Li Yilai Y Cianfrocco Michael A MA
IUCrJ 20201029 Pt 6
Recent advances in single-particle cryo-electron microscopy (cryo-EM) data collection utilize beam-image shift to improve throughput. Despite implementation on 300 keV cryo-EM instruments, it remains unknown how well beam-image-shift data collection affects data quality on 200 keV instruments and the extent to which aberrations can be computationally corrected. To test this, a cryo-EM data set for aldolase was collected at 200 keV using beam-image shift and analyzed. This analysis shows that the ...[more]