Virome Sequencing of the Human Intestinal Mucosal-Luminal Interface.
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ABSTRACT:
SUBMITTER: Yan A
PROVIDER: S-EPMC7642909 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
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Virome Sequencing of the Human Intestinal Mucosal-Luminal Interface.
Yan Austin A Butcher James J Mack David D Stintzi Alain A
Frontiers in cellular and infection microbiology 20201022
While the human gut virome has been increasingly explored in recent years, nearly all studies have been limited to fecal sampling. The mucosal-luminal interface has been established as a viable sample type for profiling the microbial biogeography of the gastrointestinal tract. We have developed a protocol to extract nucleic acids from viruses at the mucosal-luminal interface of the proximal and distal colon. Colonic viromes from pediatric patients with Crohn's disease demonstrated high interpati ...[more]
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