Ontology highlight
ABSTRACT:
SUBMITTER: Filippova IY
PROVIDER: S-EPMC7643032 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Filippova Irina Y IY Dvoryakova Elena A EA Sokolenko Nikolay I NI Simonyan Tatiana R TR Tereshchenkova Valeriia F VF Zhiganov Nikita I NI Dunaevsky Yakov E YE Belozersky Mikhail A MA Oppert Brenda B Elpidina Elena N EN
Frontiers in molecular biosciences 20201022
New substrates with glutamine in the P1-position are introduced for the assay of peptidases from the C1 papain family, with a general formula of Glp-Phe-Gln-X, where Glp is pyroglutamyl and X is pNA (<i>p</i>-nitroanilide) or AMC (4-amino-7-methylcoumaride). The substrates have a simple structure, and C1 cysteine peptidases of various origins cleave them with high efficiency. The main advantage of the substrates is their selectivity for cysteine peptidases of the C1 family. Peptidases of other c ...[more]