Ontology highlight
ABSTRACT:
SUBMITTER: Zhou H
PROVIDER: S-EPMC7644286 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Zhou Han H Cheung Jenny W JW Carpenter Tomaya T Jones Stacey K SK Luong Nhu H NH Tran Nhi C NC Jacobs Savannah E SE Galbada Liyanage Sahan A SA Cropp T Ashton TA Yin Jun J
Bioorganic & medicinal chemistry letters 20191204 2
We have improved the incorporation of l- and d-forms of unnatural amino acid (UAA) N<sup>ε</sup>-thiaprolyl-l-lysine (ThzK) into ubiquitin (UB) and green fluorescent protein (GFP) by 2-6 folds with the use of the methylester forms of the UAAs in E coli cell culture. We also improved the yields of UAA-incorporated UB and GFP with the methylester forms of N<sup>ε</sup>-Boc-l-Lysine (BocK) and N<sup>ε</sup>-propargyl-l-Lysine (PrK) by 2-5 folds compared to their free acid forms. Our work demonstrat ...[more]