Ontology highlight
ABSTRACT:
SUBMITTER: Li L
PROVIDER: S-EPMC7644725 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Li Liang L Ding Zhan Z Pang Ting-Lin TL Zhang Bei B Li Chen-Hui CH Liang An-Min AM Wang Yu-Ru YR Zhou Yu Y Fan Yu-Jie YJ Xu Yong-Zhen YZ
Nature communications 20201105 1
The minor spliceosome is evolutionarily conserved in higher eukaryotes, but its biological significance remains poorly understood. Here, by precise CRISPR/Cas9-mediated disruption of the U12 and U6atac snRNAs, we report that a defective minor spliceosome is responsible for spinal muscular atrophy (SMA) associated phenotypes in Drosophila. Using a newly developed bioinformatic approach, we identified a large set of minor spliceosome-sensitive splicing events and demonstrate that three sensitive i ...[more]