Ontology highlight
ABSTRACT:
SUBMITTER: Chiem K
PROVIDER: S-EPMC7646048 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Chiem Kevin K Ye Chengjin C Martinez-Sobrido Luis L
Current protocols in microbiology 20201201 1
SARS-CoV-2, the causative agent of COVID-19, has been responsible for a million deaths worldwide as of September 2020. At the time of this writing, there are no available US FDA-approved therapeutics for the treatment of SARS-CoV-2 infection. Here, we describe a detailed protocol to generate recombinant (r)SARS-CoV-2 using reverse-genetics approaches based on the use of a bacterial artificial chromosome (BAC). This method will allow the production of mutant rSARS-CoV-2-which is necessary for und ...[more]