DPHL: A DIA Pan-human Protein Mass Spectrometry Library for Robust Biomarker Discovery.

ABSTRACT: To address the increasing need for detecting and validating protein biomarkers in clinical specimens, mass spectrometry (MS)-based targeted proteomic techniques, including the selected reaction monitoring (SRM), parallel reaction monitoring (PRM), and massively parallel data-independent acquisition (DIA), have been developed. For optimal performance, they require the fragment ion spectra of targeted peptides as prior knowledge. In this report, we describe a MS pipeline and spectral resource to support targeted proteomics studies for human tissue samples. To build the spectral resource, we integrated common open-source MS computational tools to assemble a freely accessible computational workflow based on Docker. We then applied the workflow to generate DPHL, a comprehensive DIA pan-human li

SUBMITTER: Zhu T 

PROVIDER: S-EPMC7646093 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature