Analysis of RNA yield in extracellular vesicles isolated by membrane affinity column and differential ultracentrifugation.

ABSTRACT: Extracellular vesicles (EV) have attracted much attention as potential biomarkers due to their protein, RNA and other nucleic acid content. The most common method used for EV isolation is differential ultracentrifugation (DU), however given the DU technical difficulties, other more practical methods have surged, such as membrane-affinity column commercial kits. Here, we assessed one commercial kit in terms of EV recovery and EV-derived RNA yield and compared it with a DU protocol. Our data shows that the commercial kit preparation results in a lower count of EV-like structures and a reduced expression of EV markers when compared to DU samples. Thus, apparently suggesting that the commercial kit had a lower EV yield. However, these findings did not reflect on RNA yield, which was greater wi

SUBMITTER: Gutierrez Garcia G 

PROVIDER: S-EPMC7647092 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature