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A single-cell atlas and lineage analysis of the adult Drosophila ovary.

ABSTRACT: The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build a comprehensive cell atlas of the adult Drosophila ovary that contains transcriptional profiles for every major cell type in the ovary, including the germline stem cells and their niche cells, follicle stem cells, and previously undescribed subpopulations of escort cells. In addition, we identify Gal4 lines with specific expression patterns and perform lineage tracing of subpopulations of escort cells and follicle cells. We discover that a distinct subpopulation of escort cells is able to convert to follicle stem cells in response to starvation or upon genetic manipulation, including knockdown of escargot, or overactivation of mTor or Toll signalling.

SUBMITTER: Rust K

PROVIDER: S-EPMC7648648 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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A single-cell atlas and lineage analysis of the adult Drosophila ovary.

Rust Katja K Byrnes Lauren E LE Yu Kevin Shengyang KS Park Jason S JS Sneddon Julie B JB Tward Aaron D AD Nystul Todd G TG

Nature communications 20201106 1

The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build a comprehensive cell atlas of the adult Drosophila ovary that contains transcriptional profiles for every major cell type in the ovary, including the germline stem cells and their niche cells, follicle stem cells, and previously undescribed subpopulations of escort cells. In addition, we identify Gal4 lines with specific expression patterns and perform ...[more]

PMID: 33159074

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