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Deciphering cell-cell interactions and communication from gene expression.

ABSTRACT: Cell-cell interactions orchestrate organismal development, homeostasis and single-cell functions. When cells do not properly interact or improperly decode molecular messages, disease ensues. Thus, the identification and quantification of intercellular signalling pathways has become a common analysis performed across diverse disciplines. The expansion of protein-protein interaction databases and recent advances in RNA sequencing technologies have enabled routine analyses of intercellular signalling from gene expression measurements of bulk and single-cell data sets. In particular, ligand-receptor pairs can be used to infer intercellular communication from the coordinated expression of their cognate genes. In this Review, we highlight discoveries enabled by analyses of cell-cell interactions from transcriptomic data and review the methods and tools used in this context.

SUBMITTER: Armingol E

PROVIDER: S-EPMC7649713 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Deciphering cell-cell interactions and communication from gene expression.

Armingol Erick E Officer Adam A Harismendy Olivier O Lewis Nathan E NE

Nature reviews. Genetics 20201109 2

Cell-cell interactions orchestrate organismal development, homeostasis and single-cell functions. When cells do not properly interact or improperly decode molecular messages, disease ensues. Thus, the identification and quantification of intercellular signalling pathways has become a common analysis performed across diverse disciplines. The expansion of protein-protein interaction databases and recent advances in RNA sequencing technologies have enabled routine analyses of intercellular signalli ...[more]

PMID: 33168968

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Project description:A number of factors have been investigated in the context of gene function prediction and analysis, such as sequence identity, gene expressions, and gene co-evolution. However, three-dimensional (3D) conformation of the genome has not been tapped to analyse gene function, probably largely due to lack of genome conformation data until recently.We construct the genome-wide spatial gene-gene interaction networks for three different human B-cells or cell lines from their chromosomal contact data generated by the Hi-C chromosome conformation capturing technique. The G-SESAME and Fast-SemSim are used to calculate function similarity between interacted / non-interacted genes. The Gene Ontology statistics computed from the gene-gene interaction networks is used for gene function prediction.We compare the function similarity of gene pairs that do not spatially interact and that have interactions. We find that genes that have strong spatial interactions tend to have highly similar function in terms of biological process, molecular function and cellular component of the Gene Ontology. And even though the level of gene-gene interactions generally have no or weak correlation with either sequential genomic distance or sequence identity between genes, the interacted genes with high function similarity tend to have stronger interactions, somewhat shorter genomic distance and significantly higher sequence identity. And combining genomic distance or sequence identity with spatial gene-gene interaction information informs gene-gene function similarity much better than using either one of them alone, suggesting gene-gene interaction information is largely complementary with genomic distance and sequence identity in the context of gene function analysis. We develop and evaluate a new gene function prediction method based on gene-gene interacting networks, which can predict gene function well for a large number of human genes.In this work, we demonstrate that the spatial conformation of the human genome is relevant to gene function similarity and is useful for gene function prediction.

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Dataset Information

Deciphering cell-cell interactions and communication from gene expression.

Publications

Deciphering cell-cell interactions and communication from gene expression.

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