Ontology highlight
ABSTRACT:
SUBMITTER: Roush AE
PROVIDER: S-EPMC7650270 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Roush Addison E AE Riaz Mohammad M Misra Sandeep K SK Weinberger Scot R SR Sharp Joshua S JS
Journal of the American Society for Mass Spectrometry 20191218 2
Fast photochemical oxidation of proteins (FPOP) is a powerful covalent labeling tool that uses hydroxyl radicals generated by laser flash photolysis of hydrogen peroxide to footprint protein surfaces. Because radical production varies with many experimental parameters, hydroxyl radical dosimeters have been introduced to track the effective radical dosage experienced by the protein analyte. FPOP experiments performed using adenine optical radical dosimetry containing protein in Tris buffer demons ...[more]