Dataset Information

Effect of Molecular Weight on Gelling and Viscoelastic Properties of Poly(caprolactone)-b-Poly(ethylene glycol)-b-Poly(caprolactone) (PCL-PEG-PCL) Hydrogels.

ABSTRACT: Hydrogels based on poly(caprolactone)-b-poly(ethylene glycol)-b-poly(caprolactone) (PCL-PEG-PCL) have been evaluated extensively as potential injectable fillers or depots for controlled release of drugs. Common drawbacks of these copolymer systems include instability of aqueous solutions and low mechanical strength of gels, issues which are commonly overcome by adding pendant groups to the end of the copolymer chains. Here, a systematic study of the effects of increasing polymer molecular weight (MW) is presented, utilizing PEG blocks of MW 2, 4 or 8 kDa. Triblock copolymers were prepared by the ring-opening polymerization of ?-caprolactone by PEG. Copolymers prepared with PEG MW 2 kDa did not form hydrogels at any copolymer molecular weight. Copolymers prepared with PEG MW 4 kDa formed gels at MW between 11 and 13.5 kDa, and copolymers prepared with PEG MW 8 kDa formed gels at MW between 16 and 18 kDa. Copolymers with PEG block 8 kDa formed hydrogels with high viscosity (17,000 Pa·s) and mechanical strength (G' = 14,000 Pa). The increased gel strength afforded by increased molecular weight represents a simple modification of the reactants used in the reaction feed without added synthetic or purification steps. Shear-thinning of PCL-PEG-PCL triblock copolymer hydrogels allowed for injection through a standard 23G syringe, allowing for potential use as dermal fillers or drug delivery depots.

SUBMITTER: Steinman NY

PROVIDER: S-EPMC7650642 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Effect of Molecular Weight on Gelling and Viscoelastic Properties of Poly(caprolactone)-b-Poly(ethylene glycol)-b-Poly(caprolactone) (PCL-PEG-PCL) Hydrogels.

Steinman Noam Y NY Bentolila Noam Y NY Domb Abraham J AJ

Polymers 20201015 10

Hydrogels based on poly(caprolactone)-<i>b</i>-poly(ethylene glycol)-<i>b</i>-poly(caprolactone) (PCL-PEG-PCL) have been evaluated extensively as potential injectable fillers or depots for controlled release of drugs. Common drawbacks of these copolymer systems include instability of aqueous solutions and low mechanical strength of gels, issues which are commonly overcome by adding pendant groups to the end of the copolymer chains. Here, a systematic study of the effects of increasing polymer mo ...[more]

PMID: 33076459

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Project description:Growth factors have been shown to be potent mediators of osteogenesis. However, their use in tissue-engineered scaffolds not only can be costly but also can induce undesired responses in surrounding tissues. Thus, the ability to specifically induce osteogenic differentiation in the absence of exogenous growth factors through manipulation of scaffold material properties would be desirable for bone regeneration. Previous research indicates that addition of inorganic or hydrophobic components to organic, hydrophilic scaffolds can enhance multipotent stem cell (MSC) osteogenesis. However, the combined impact of scaffold inorganic content and hydrophobicity on MSC behavior has not been systematically explored, particularly in three-dimensional (3D) culture systems. The aim of the present study was therefore to examine the effects of simultaneous increases in scaffold hydrophobicity and inorganic content on MSC osteogenic fate decisions in a 3D culture environment toward the development of intrinsically osteoinductive scaffolds. Mouse 10T½ MSCs were encapsulated in a series of novel scaffolds composed of varying levels of hydrophobic, inorganic poly(dimethylsiloxane) (PDMS) and hydrophilic, organic poly(ethylene glycol) (PEG). After 21 days of culture, increased levels of osteoblast markers, runx2 and osteocalcin, were observed in scaffolds with increased PDMS content. Bone extracellular matrix (ECM) molecules, collagen I and calcium phosphate, were also elevated in formulations with higher PDMS:PEG ratios. Importantly, this osteogenic response appeared to be specific in that markers for chondrocytic, smooth muscle cell, and adipocytic lineages were not similarly affected by variations in scaffold PDMS content. As anticipated, the increase in scaffold hydrophobicity accompanying increasing PDMS levels was associated with elevated scaffold serum protein adsorption. Thus, scaffold inorganic content combined with alterations in adsorbed serum proteins may underlie the observed cell behavior.

Dataset Information

Effect of Molecular Weight on Gelling and Viscoelastic Properties of Poly(caprolactone)-b-Poly(ethylene glycol)-b-Poly(caprolactone) (PCL-PEG-PCL) Hydrogels.

Publications

Effect of Molecular Weight on Gelling and Viscoelastic Properties of Poly(caprolactone)-b-Poly(ethylene glycol)-b-Poly(caprolactone) (PCL-PEG-PCL) Hydrogels.

Similar Datasets

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