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An Easy Method for Pressure Measurement in Microchannels Using Trapped Air Compression in a One-End-Sealed Capillary.

ABSTRACT: Pressure is one basic parameter involved in microfluidic systems. In this study, we developed an easy capillary-based method for measuring fluid pressure at one or multiple locations in a microchannel. The principal component is a commonly used capillary (inner diameter of 400 ?m and 95 mm in length), with one end sealed and calibrated scales on it. By reading the height (h) of an air-liquid interface, the pressure can be measured directly from a table, which is calculated using the ideal gas law. Many factors that affect the relationship between the trapped air volume and applied pressure (papplied) have been investigated in detail, including the surface tension, liquid gravity, air solubility in water, temperature variation, and capillary diameters. Based on the evaluation of the experimental and simulation results of the pressure, combined with theoretical analysis, a resolution of about 1 kPa within a full-scale range of 101.6-178 kPa was obtained. A pressure drop (?p) as low as 0.25 kPa was obtained in an operating range from 0.5 kPa to 12 kPa. Compared with other novel, microstructure-based methods, this method does not require microfabrication and additional equipment. Finally, we use this method to reasonably analyze the nonlinearity of the flow-pressure drop relationship caused by channel deformation. In the future, this one-end-sealed capillary could be used for pressure measurement as easily as a clinical thermometer in various microfluidic applications.

SUBMITTER: Shen F

PROVIDER: S-EPMC7650790 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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An Easy Method for Pressure Measurement in Microchannels Using Trapped Air Compression in a One-End-Sealed Capillary.

Shen Feng F Ai Mingzhu M Ma Jianfeng J Li Zonghe Z Xue Sen S

Micromachines 20200930 10

Pressure is one basic parameter involved in microfluidic systems. In this study, we developed an easy capillary-based method for measuring fluid pressure at one or multiple locations in a microchannel. The principal component is a commonly used capillary (inner diameter of 400 μm and 95 mm in length), with one end sealed and calibrated scales on it. By reading the height (<i>h</i>) of an air-liquid interface, the pressure can be measured directly from a table, which is calculated using the ideal ...[more]

PMID: 33008031

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Project description:Pulmonary hypoplasia is a condition of the newborn that is characterized by underdeveloped lungs and poor outcome. One strategy in the treatment of patients with hypoplasia is to augment underdeveloped lungs using biocompatible artificial lung tissue. However, one central challenge in current pulmonary tissue engineering efforts remains the development of a stable bio-mimetic alveolar-capillary membrane. Accordingly, we have built a series of bio-mimetic microfluidic devices that specifically model the alveolar-capillary membrane. Current designs include a single-layer microchip that exposes alveolar and endothelial cell types to controlled fluidic stimuli. A more advanced multi-layered device allows for alveolar cells to be cultured at an air interface while allowing constant media nourishment and waste removal, thus better mimicking the physiologic milieu of the alveolar-capillary interface. Both devices possess the benefit of parallel testing.Microdevices were fabricated using soft lithography in a biocompatible transparent polymeric material, polydimethyl siloxane, sealed covalently to glass. The multistage microdevice also integrated a suspended polyethylene terephthalate membrane connected via microfluidic channels to constant media and air access. Pulmonary endothelial (HMEC-1) and alveolar epithelial (A549) cell lines, along with fetal pulmonary cells (FPC) harvested from Swiss Webster mice at day 18 gestational age, were studied under multiple hydrodynamic shear conditions and liquid-to-cell ratio regimes. Cultures were examined for cell viability, function and proliferation to confluent monolayers. A549 cells cultured at an air-interface in a microdevice was also tested for their ability to maintain cell phenotype and function.The single-layer differential flow microdevice allowed for a systematic determination of the optimal growth conditions of various lung-specific cell types in a microfluidic environment. Our device showed a greater surfactant based decrease in surface tension of the alveolar hypophase in A549 cultures exposed to air as compared to submerged cultures.We have successfully developed biomimetic microfluidic devices that specifically allow stable alveolar cell growth at the air-liquid interface. This work serves prerequisite towards an implantable artificial alveolar membrane.

Dataset Information

An Easy Method for Pressure Measurement in Microchannels Using Trapped Air Compression in a One-End-Sealed Capillary.

Publications

An Easy Method for Pressure Measurement in Microchannels Using Trapped Air Compression in a One-End-Sealed Capillary.

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