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Deep Sequencing Identified Dysregulated Circulating MicroRNAs in Late Onset Preeclampsia.

ABSTRACT: BACKGROUND/AIM:To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop late-onset preeclampsia (LOPE) compared to uncomplicated pregnancies. MATERIALS AND METHODS:Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for confirmation in an independent cohort of 12 LOPE cases and 12 controls. RESULTS:miR-23b-5p and miR-99b-5p were down-regulated by >1.5 fold in LOPE complicated pregnancies (p value <0.05) compared to controls. Target prediction showed that the major targets of these miRNAs are associated with glycometabolism and immune response. CONCLUSION:miR-23b-5p and miR-99b-5p are possibly implicated in the pathogenic mechanisms leading to the induction of LOPE and may serve as candidate non-invasive biomarkers for early prediction and prevention.

SUBMITTER: Mavreli D 

PROVIDER: S-EPMC7652460 | biostudies-literature | 2020 Sep-Oct

REPOSITORIES: biostudies-literature

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Deep Sequencing Identified Dysregulated Circulating MicroRNAs in Late Onset Preeclampsia.

Mavreli Danai D   Lykoudi Alexandra A   Lambrou George G   Papaioannou George G   Vrachnis Nikolas N   Kalantaridou Sophia S   Papantoniou Nikolas N   Kolialexi Aggeliki A  

In vivo (Athens, Greece) 20200901 5

<h4>Background/aim</h4>To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop late-onset preeclampsia (LOPE) compared to uncomplicated pregnancies.<h4>Materials and methods</h4>Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-t  ...[more]

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