Unknown

Dataset Information

0

The association between SA?2,3Gal occurrence frequency and avian influenza viral load in mallards (Anas platyrhynchos) and blue-winged teals (Spatula discors).


ABSTRACT: BACKGROUND:Individual heterogeneity in pathogen load can affect disease transmission dynamics; therefore, identifying intrinsic factors responsible for variation in pathogen load is necessary for determining which individuals are prone to be most infectious. Because low pathogenic avian influenza viruses (LPAIV) preferentially bind to alpha-2,3 sialic acid receptors (SA?2,3Gal) in the intestines and bursa of Fabricius in wild ducks (Anas and Spatula spp.), we investigated juvenile mallards (Anas platyrhyncos) and blue-winged teals (Anas discors) orally inoculated with A/northern pintail/California/44221-761/2006 (H5N9) and the virus titer relationship to occurrence frequency of SA?2,3Gal in the intestines and bursa. To test the natural variation of free-ranging duck populations, birds were hatched and raised in captivity from eggs collected from nests of free-ranging birds in North Dakota, USA. Data generated from qPCR were used to quantify virus titers in cloacal swabs, ileum tissue, and bursa of Fabricius tissue, and lectin histochemistry was used to quantify the occurrence frequency of SA?2,3Gal. Linear mixed models were used to analyze infection status, species, and sex-based differences. Multiple linear regression was used to analyze the relationship between virus titer and SA?2,3Gal occurrence frequency. RESULTS:In mallards, we found high individual variation in virus titers significantly related to high variation of SA?2,3Gal in the ileum. In contrast to mallards, individual variation in teals was minimal and significant relationships between virus titers and SA?2,3Gal were not determined. Collectively, teals had both higher virus titers and a higher occurrence frequency of SA?2,3Gal compared to mallards, which may indicate a positive association between viral load and SA?2,3Gal. Statistically significant differences were observed between infected and control birds indicating that LPAIV infection may influence the occurrence frequency of SA?2,3Gal, or vice versa, but only in specific tissues. CONCLUSIONS:The results of this study provide quantitative evidence that SA?2,3Gal abundance is related to LPAIV titers; thus, SA?2,3Gal should be considered a potential intrinsic factor influencing variation in LPAIV load.

SUBMITTER: Dolinski AC 

PROVIDER: S-EPMC7653716 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

The association between SAα2,3Gal occurrence frequency and avian influenza viral load in mallards (Anas platyrhynchos) and blue-winged teals (Spatula discors).

Dolinski Amanda C AC   Jankowski Mark D MD   Fair Jeanne M JM   Owen Jennifer C JC  

BMC veterinary research 20201110 1


<h4>Background</h4>Individual heterogeneity in pathogen load can affect disease transmission dynamics; therefore, identifying intrinsic factors responsible for variation in pathogen load is necessary for determining which individuals are prone to be most infectious. Because low pathogenic avian influenza viruses (LPAIV) preferentially bind to alpha-2,3 sialic acid receptors (SAα2,3Gal) in the intestines and bursa of Fabricius in wild ducks (Anas and Spatula spp.), we investigated juvenile mallar  ...[more]

Similar Datasets

| S-EPMC4227685 | biostudies-literature
| S-EPMC7106234 | biostudies-literature
| S-EPMC10519146 | biostudies-literature
| S-EPMC7115110 | biostudies-literature
| S-EPMC4757037 | biostudies-literature
| S-EPMC4006863 | biostudies-literature
| S-EPMC7354581 | biostudies-literature
| S-EPMC9964785 | biostudies-literature
| S-EPMC5570339 | biostudies-literature
| S-EPMC3335010 | biostudies-literature