Ontology highlight
ABSTRACT:
SUBMITTER: Ooi LC
PROVIDER: S-EPMC7654252 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Ooi Li-Ching LC Watanabe Nobumoto N Futamura Yushi Y Sulaiman Shaida Fariza SF Darah Ibrahim I Osada Hiroyuki H
Cancer science 20130906 11
Dysregulation of p27(Kip1) due to proteolysis that involves the ubiquitin ligase (SCF) complex with S-phase kinase-associated protein 2 (Skp2) as the substrate-recognition component (SCF(Skp2)) frequently results in tumorigenesis. In this report, we developed a high-throughput screening system to identify small-molecule inhibitors of p27(Kip1) degradation. This system was established by tagging Skp2 with fluorescent monomeric Azami Green (mAG) and CDK subunit 1 (Cks1) (mAGSkp2-Cks1) to bind to p ...[more]