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Impact of chronic endometritis on endometrial receptivity analysis results and pregnancy outcomes.

ABSTRACT: BACKGROUND:The aim of this study is to evaluate the relationship between chronic endometritis (CE) and a personalized window of implantation (WOI), identified by results of endometrial receptivity analysis (ERA), and pregnancy outcomes following embryo transfer (ET) based on the ERA outcomes. METHODS:The single-center, cross-sectional study was designed. The study population consisted of 101 infertile women who underwent endometrial sampling between June 2018 and February 2020. We recruited 88 patients who underwent ERA testing and immunohistochemistry of the plasma cell marker CD138 to diagnose CE within 3 months of testing. Subjects were divided into three groups as follows: 33 without CE (non-CE group); 19 with untreated CE at ERA testing (CE group); and 36 successfully treated for CE before ERA testing (cured-CE group). CE diagnosis was defined as ?5 CD138-positive plasma cells per 10 random stromal areas at ×400 magnification. RESULTS:In non-CE, CE, and cured-CE groups, the numbers of CD138-positive cells were 0.7?±?1.0, 28.5?±?30.4, and 1.3?±?1.3, respectively (p?

SUBMITTER: Kuroda K

PROVIDER: S-EPMC7654412 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Impact of chronic endometritis on endometrial receptivity analysis results and pregnancy outcomes.

Kuroda Keiji K Horikawa Takashi T Moriyama Azusa A Nakao Kazuki K Juen Hiroyasu H Takamizawa Satoru S Ojiro Yuko Y Nakagawa Koji K Sugiyama Rikikazu R

Immunity, inflammation and disease 20200923 4

<h4>Background</h4>The aim of this study is to evaluate the relationship between chronic endometritis (CE) and a personalized window of implantation (WOI), identified by results of endometrial receptivity analysis (ERA), and pregnancy outcomes following embryo transfer (ET) based on the ERA outcomes.<h4>Methods</h4>The single-center, cross-sectional study was designed. The study population consisted of 101 infertile women who underwent endometrial sampling between June 2018 and February 2020. We ...[more]

PMID: 32969185

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Project description:Purpose:Adenomyosis has a negative impact on female fertility. GnRH agonist treatment can improve pregnancy outcomes in women with adenomyosis. However, the impact of GnRH agonist upon endometrium receptivity of patients with adenomyosis remains unclear. In this study, endometrial receptivity and pregnancy outcome were investigated using a mouse model of adenomyosis. Materials and methods:Adenomyosis was induced in 12 female ICR mice, neonatally treated with tamoxifen, while another six female mice (control group) received solvent only. At 75 days, the induced adenomyosis group was randomly divided into two groups: an untreated group and a group treated with GnRH agonist (n = 6 each). Sixty days later, the mice were mated and pregnancy outcomes were observed and compared among the three groups (n = 6 each). In a parallel experiment using the same treatment regimes, uterus samples were collected on day 4 of pregnancy for immunohistochemistry, gene (quantitative polymerase chain reaction) and protein expression (Western blot), and scanning electron microscopy analyses. Results:We found that the average live litter size was reduced in the adenomyosis compared with control group (8 ± 0.56 versus 13 ± 0.71; P = 0.03). However, the litter size was significantly increased in the treated with GnRH agonist group compared with the untreated group (12 ± 0.35 versus 8 ± 0.56; P = 0.04). The uterine expression levels of Hoxa10, Hoxa11, Lif and integrin b3 mRNA and protein were decreased in the adenomyosis group, and were significantly increased after GnRH agonist treatment. Additionally, pinopodes were reduced in number and poorly developed in mice with induced adenomyosis. However, pinopodes were abundant and well-developed in the GnRH agonist treatment group. Conclusion:Adenomyosis may have an adverse impact on endometrial receptivity and reduce pregnancy outcomes in mice. However, GnRH agonist may improve the pregnancy outcome by partially restoring endometrial receptivity.

Dataset Information

Impact of chronic endometritis on endometrial receptivity analysis results and pregnancy outcomes.

Publications

Impact of chronic endometritis on endometrial receptivity analysis results and pregnancy outcomes.

Similar Datasets

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