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Novel enzymatic elimination method for the chromatographic purification of ginsenoside Rb3 in an isomeric mixture.


ABSTRACT: Background:The separation of isomeric compounds from a mixture is a recurring problem in chemistry and phytochemistry research. The purification of pharmacologically active ginsenoside Rb3 from ginseng extracts is limited by the co-existence of its isomer Rb2. The aim of the present study was to develop an enzymatic elimination-combined purification method to obtain pure Rb3 from a mixture of isomers. Methods:To isolate Rb3 from the isomeric mixture, a simple enzymatic selective elimination method was used. A ginsenoside-transforming glycoside hydrolase (Bgp2) was employed to selectively hydrolyze Rb2 into ginsenoside Rd. Ginsenoside Rb3 was then efficiently separated from the mixture using a traditional chromatographic method. Results:Chromatographic purification of Rb3 was achieved using this novel enzymatic elimination-combined method, with 58.6-times higher yield and 13.1% less time than those of the traditional chromatographic method, with a lower minimum column length for purification. The novelty of this study was the use of a recombinant glycosidase for the selective elimination of the isomer. The isolated ginsenoside Rb3 can be used in further pharmaceutical studies. Conclusions:Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb3. This method can also be applied to purify other isomeric glycoconjugates in mixtures.

SUBMITTER: Cui CH 

PROVIDER: S-EPMC7655484 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Novel enzymatic elimination method for the chromatographic purification of ginsenoside Rb<sub>3</sub> in an isomeric mixture.

Cui Chang-Hao CH   Fu Yaoyao Y   Jeon Byeong-Min BM   Kim Sun-Chang SC   Im Wan-Taek WT  

Journal of ginseng research 20190817 6


<h4>Background</h4>The separation of isomeric compounds from a mixture is a recurring problem in chemistry and phytochemistry research. The purification of pharmacologically active ginsenoside Rb<sub>3</sub> from ginseng extracts is limited by the co-existence of its isomer Rb<sub>2</sub>. The aim of the present study was to develop an enzymatic elimination-combined purification method to obtain pure Rb<sub>3</sub> from a mixture of isomers.<h4>Methods</h4>To isolate Rb<sub>3</sub> from the isom  ...[more]

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