Unknown

Dataset Information

0

Immunoinformatic Analysis of SARS-CoV-2 Nucleocapsid Protein and Identification of COVID-19 Vaccine Targets.


ABSTRACT: COVID-19 is a worldwide emergency; therefore, there is a critical need for foundational knowledge about B and T cell responses to SARS-CoV-2 essential for vaccine development. However, little information is available defining which determinants of SARS-CoV-2 other than the spike glycoprotein are recognized by the host immune system. In this study, we focus on the SARS-CoV-2 nucleocapsid protein as a suitable candidate target for vaccine formulations. Major B and T cell epitopes of the SARS-CoV-2 N protein are predicted and resulting sequences compared with the homolog immunological domains of other coronaviruses that infect human beings. The most dominant of B cell epitope is located between 176-206 amino acids in the SRGGSQASSRSSSRSRNSSRNSTPGSSRGTS sequence. Further, we identify sequences which are predicted to bind multiple common MHC I and MHC II alleles. Most notably there is a region of potential T cell cross-reactivity within the SARS-CoV-2 N protein position 102-110 amino acids that traverses multiple human alpha and betacoronaviruses. Vaccination strategies designed to target these conserved epitope regions could generate immune responses that are cross-reactive across human coronaviruses, with potential to protect or modulate disease. Finally, these predictions can facilitate effective vaccine design against this high priority virus.

SUBMITTER: Oliveira SC 

PROVIDER: S-EPMC7655779 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

Immunoinformatic Analysis of SARS-CoV-2 Nucleocapsid Protein and Identification of COVID-19 Vaccine Targets.

Oliveira Sergio C SC   de Magalhães Mariana T Q MTQ   Homan E Jane EJ  

Frontiers in immunology 20201028


COVID-19 is a worldwide emergency; therefore, there is a critical need for foundational knowledge about B and T cell responses to SARS-CoV-2 essential for vaccine development. However, little information is available defining which determinants of SARS-CoV-2 other than the spike glycoprotein are recognized by the host immune system. In this study, we focus on the SARS-CoV-2 nucleocapsid protein as a suitable candidate target for vaccine formulations. Major B and T cell epitopes of the SARS-CoV-2  ...[more]

Similar Datasets

| S-SCDT-EMM-2022-15904 | biostudies-other
| S-EPMC7150947 | biostudies-literature
| S-EPMC7704662 | biostudies-literature
| S-EPMC9315333 | biostudies-literature
| S-EPMC7351549 | biostudies-literature
2021-04-09 | GSE171668 | GEO
| S-EPMC8623253 | biostudies-literature
| S-EPMC7241347 | biostudies-literature
| S-EPMC8762837 | biostudies-literature
| S-EPMC8636620 | biostudies-literature