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Metabolic changes in the developing sugarcane culm associated with high yield and early high sugar content.

ABSTRACT: Sugarcane, with its exceptional biomass and sugar yield, has a high potential for the production of bioenergy, biomaterials, and high-value products. Currently, the link between metabolic changes in the developing internodes in sugarcane and final yield and sugar characteristics is not well understood. In order to investigate these correlations, 1,440 internodes were collected and combined to generate a set of 360 samples across 24 sugarcane cultivars at five different developmental stages. A combination of metabolome profiling and trait co-expression analyses were conducted to reveal the interaction between the metabolome and essential agronomic traits, including Brix (total sugar), polarity (sucrose content), purity (sucrose purity), commercially extractable sucrose, fiber, and tons of cane per hectare (TCH). Metabolomic analysis revealed significant differences in metabolic patterns mainly correlated with developmental stage. Hierarchical clustering of genotypes and traits revealed clear partitioning of groups of early-, mid- and late-season sugar content, with secondary segregation by the yield trait, TCH, and fiber content. The study identified co-expression and specific metabolites associated with metabolic pathways correlated with Brix and fiber content. Knowledge of the correlation between co-expressed metabolites and diverse agronomic traits will allow more deliberate selection of genotypes for early or late sugar development and fiber content and biomass yield.

SUBMITTER: Perlo V

PROVIDER: S-EPMC7656173 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Metabolic changes in the developing sugarcane culm associated with high yield and early high sugar content.

Perlo Virginie V Botha Frederik C FC Furtado Agnelo A Hodgson-Kratky Katrina K Henry Robert J RJ

Plant direct 20201111 11

Sugarcane, with its exceptional biomass and sugar yield, has a high potential for the production of bioenergy, biomaterials, and high-value products. Currently, the link between metabolic changes in the developing internodes in sugarcane and final yield and sugar characteristics is not well understood. In order to investigate these correlations, 1,440 internodes were collected and combined to generate a set of 360 samples across 24 sugarcane cultivars at five different developmental stages. A co ...[more]

PMID: 33204934

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Project description:Sugarcane is a major crop of the tropics cultivated mainly for its high sucrose content. The crop is genetically less explored due to its complex polyploid genome. Sucrose synthesis and accumulation are complex processes influenced by physiological, biochemical and genetic factors, and the growth environment. The recent focus on the crop for fibre and biofuel has led to a renewed interest on understanding the molecular basis of sucrose and biomass traits. This transcriptome study aimed to identify genes that are associated with and differentially regulated during sucrose synthesis and accumulation in the mature stage of sugarcane. Patterns of gene expression in high and low sugar genotypes as well as mature and immature culm tissues were studied using RNA-Seq of culm transcriptomes.In this study, 28 RNA-Seq libraries from 14 genotypes of sugarcane differing in their sucrose content were used for studying the transcriptional basis of sucrose accumulation. Differential gene expression studies were performed using SoGI (Saccharum officinarum Gene Index, 3.0), SAS (sugarcane assembled sequences) of sugarcane EST database (SUCEST) and SUGIT, a sugarcane Iso-Seq transcriptome database. In total, about 34,476 genes were found to be differentially expressed between high and low sugar genotypes with the SoGI database, 20,487 genes with the SAS database and 18,543 genes with the SUGIT database at FDR?<?0.01, using the Baggerley's test. Further, differential gene expression analyses were conducted between immature (top) and mature (bottom) tissues of the culm. The DEGs were functionally annotated using GO classification and the genes consistently associated with sucrose accumulation were identified.The large number of DEGs may be due to the large number of genes that influence sucrose content or are regulated by sucrose content. These results indicate that apart from being a primary metabolite and storage and transport sugar, sucrose may serve as a signalling molecule that regulates many aspects of growth and development in sugarcane. Further studies are needed to confirm if sucrose regulates the expression of the identified DEGs or vice versa. The DEGs identified in this study may lead to identification of genes/pathways regulating sucrose accumulation and/or regulated by sucrose levels in sugarcane. We propose identifying the master regulators of sucrose if any in the future.

Dataset Information

Metabolic changes in the developing sugarcane culm associated with high yield and early high sugar content.

Publications

Metabolic changes in the developing sugarcane culm associated with high yield and early high sugar content.

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