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The secondary structural difference between Lewy body and glial cytoplasmic inclusion in autopsy brain with synchrotron FTIR micro-spectroscopy.

ABSTRACT: Lewy bodies (LBs) and glial cytoplasmic inclusions (GCIs) are specific aggregates found in Parkinson's disease (PD) and multiple system atrophy (MSA), respectively. These aggregates mainly consist of ?-synuclein (?-syn) and have been reported to propagate in the brain. In animal experiments, the fibrils of ?-syn propagate similarly to prions but there is still insufficient evidence to establish this finding in humans. Here, we analysed the protein structure of these aggregates in the autopsy brains of patients by synchrotron Fourier-transform infrared micro-spectroscopy (FTIRM) analysis without extracting or artificially amplifying the aggregates. As a result, we found that the content of the ?-sheet structure in LBs in patients with PD was significantly higher than that in GCIs in patients with MSA (52.6?±?1.9% in PD vs. 38.1?±?0.9% in MSA, P?

SUBMITTER: Araki K 

PROVIDER: S-EPMC7656264 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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The secondary structural difference between Lewy body and glial cytoplasmic inclusion in autopsy brain with synchrotron FTIR micro-spectroscopy.

Araki Katsuya K   Yagi Naoto N   Ikemoto Yuka Y   Hayakawa Hideki H   Fujimura Harutoshi H   Moriwaki Taro T   Nagai Yoshitaka Y   Murayama Shigeo S   Mochizuki Hideki H  

Scientific reports 20201110 1

Lewy bodies (LBs) and glial cytoplasmic inclusions (GCIs) are specific aggregates found in Parkinson's disease (PD) and multiple system atrophy (MSA), respectively. These aggregates mainly consist of α-synuclein (α-syn) and have been reported to propagate in the brain. In animal experiments, the fibrils of α-syn propagate similarly to prions but there is still insufficient evidence to establish this finding in humans. Here, we analysed the protein structure of these aggregates in the autopsy bra  ...[more]

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