Dataset Information

Bacteriome-Associated Endosymbiotic Bacteria of Nosodendron Tree Sap Beetles (Coleoptera: Nosodendridae).

ABSTRACT: The family Nosodendridae is a small group of tree sap beetles with only 91 described species representing three genera from the world. In 1930s, bacteria-harboring symbiotic organs, called bacteriomes, were briefly described in a European species Nosodendron fasciculare. Since then, however, no studies have been conducted on the nosodendrid endosymbiosis for decades. Here we investigated the bacteriomes and the endosymbiotic bacteria of Nosodendron coenosum and Nosodendron asiaticum using molecular phylogenetic and histological approaches. In adults and larvae, a pair of slender bacteriomes were found along both sides of the midgut. The bacteriomes consisted of large bacteriocytes at the center and flat sheath cells on the surface. Fluorescence in situ hybridization detected preferential localization of the endosymbiotic bacteria in the cytoplasm of the bacteriocytes. In reproductive adult females, the endosymbiotic bacteria were also detected at the infection zone in the ovarioles and on the surface of growing oocytes, indicating vertical symbiont transmission via ovarial passage. Transmission electron microscopy unveiled bizarre structural features of the bacteriocytes, whose cytoplasm exhibited degenerate cytology with deformed endosymbiont cells. Molecular phylogenetic analysis revealed that the nosodendrid endosymbionts formed a distinct clade in the Bacteroidetes. The nosodendrid endosymbionts were the most closely related to the bacteriome endosymbionts of bostrichid powderpost beetles and also allied to the bacteriome endosymbionts of silvanid grain beetles, uncovering an unexpected endosymbiont relationship across the unrelated beetle families Nosodendridae, Bostrichidae and Silvanidae. Host-symbiont co-evolution and presumable biological roles of the endosymbiotic bacteria are discussed.

SUBMITTER: Hirota B

PROVIDER: S-EPMC7658545 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Bacteriome-Associated Endosymbiotic Bacteria of Nosodendron Tree Sap Beetles (Coleoptera: Nosodendridae).

Hirota Bin B Meng Xian-Ying XY Fukatsu Takema T

Frontiers in microbiology 20201029

The family Nosodendridae is a small group of tree sap beetles with only 91 described species representing three genera from the world. In 1930s, bacteria-harboring symbiotic organs, called bacteriomes, were briefly described in a European species Nosodendron fasciculare. Since then, however, no studies have been conducted on the nosodendrid endosymbiosis for decades. Here we investigated the bacteriomes and the endosymbiotic bacteria of Nosodendron coenosum and Nosodendron asiat ...[more]

PMID: 33193249

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Project description:BackgroundThe date palm root borer Oryctes agamemnon (Coleoptera: Scarabaeidae) is one of the major pests of palms. In Saudi Arabia, both larvae and adults of Oryctes are particularly troublesome, especially during the establishment of young date palm orchards. Endosymbiotic bacteria are known to have a key role in food digestion and insecticide resistance mechanisms, and therefore are essential to their host insect. Identification of these bacteria in their insect host can lead to development of new insect pest control strategies.ResultsMetagenomic DNA from larval midgut of the date palm root borer, O. agamemnon, was analyzed for endosymbiotic bacterial communities using denatured gradient gel electrophoresis (DGGE) utilizing 16S rRNA genes. The DGGE fingerprints with metagenomic DNA showed predominance of eleven major operational taxonomic units (OTUs) identified as members of Photobacterium, Vibrio, Allomonas, Shewanella, Cellulomonas, and Citrobacter, as well as uncultured bacteria, including some uncultured Vibrio members. DGGE profiles also showed shifts in the dominant bacterial populations of the original soil compared with those that existed in the larval midguts. The endosymbiotic bacterial community was dominated by members of the family Vibrionaceae (54.5%), followed by uncultured bacteria (18.2%), Enterobacteriaceae (9.1%), Shewanellaceae (9.1%), and Cellulomonadaceae (9.1%). Phylogenetic studies confirmed the affiliation of the dominant OTUs into specified families revealed by clustering of each phylotype to its corresponding clade. Relative frequency of each phylotype in larval midguts revealed predominance of Vibrio furnisii and Vibrio navarrensis, followed by uncultured bacterial spp., then Cellulomonas hominis, Shewanella algae, and Citrobacter freundii.ConclusionAnalysis of metagenomic DNA for endosymbiotic bacterial communities from the midgut of Oryctes larvae showed strong selection of specific bacterial populations that may have a key role in digestion, as well as other benefits to the larvae of O. agamemnon. Determination of the distinct endosymbiotic community structure and its possible biological functions within the insect could provide us with basic information for future pest control research.

Project description:Longhorn beetles (coleopteraCerambycidae) have been used to identify sites of high biological diversity and conservation value in cultivated landscapes, but were rarely studied in changing landscapes of humid tropics. This study was conducted in a region of southern Yunnan, China, which was dominated by natural rainforest until 30 years ago, but is successively transformed into commercial rubber monoculture plantations since that time. The objectives were to investigate longhorn beetle species diversity and distribution in the major land use types of this landscape and to estimate the effects of an expected expansion of rubber plantations on the longhorn beetle assemblages. The results showed that tree species diversity (181 species in total) and longhorn beetle diversity (220 species in total) were closely related with no significant differences between the tree and longhorn beetles assemblages shown by similarity distance analysis. There was a highly positive relationship between the estimated species richness of longhorn beetles and the number of tree species. Individual numbers of longhorn beetles and trees were also highly positive related at the sampling sites. Non-metric multidimensional scaling revealed that the degree of canopy coverage, succession age and tree diversity explained 78.5% of the total variation in longhorn beetle assemblage composition. Natural forest sites had significantly higher numbers of species and individuals than any other type of habitat. Although young rubber plantations bear the highest longhorn beetle diversity outside forests (half of the total number of longhorn beetle species recorded in total), they can not provide permanent habitats for most of these species, because they develop into closed canopy plantations with less suitable habitat conditions. Therefore, along with an expected expansion of rubber cultivation which largely proceeds at the expense of forest areas, the habitat conditions for longhorn beetles in this region might decrease dramatically in future.

Dataset Information

Bacteriome-Associated Endosymbiotic Bacteria of Nosodendron Tree Sap Beetles (Coleoptera: Nosodendridae).

Publications

Bacteriome-Associated Endosymbiotic Bacteria of <i>Nosodendron</i> Tree Sap Beetles (Coleoptera: Nosodendridae).

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