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Short DNA Oligonucleotide as a Ag+ Binding Detector.


ABSTRACT: Ag+ has been known to mediate several natural metallo-base pairs. Based on the unique structural information of a short 8-mer DNA strand (5'-GCACGCGC-3') induced by Ag+, we constructed several fluorescent DNA beacons for the detection of Ag+ according to the increase in the fluorescence emission on Ag+ binding. This Ag+ detection assay is quick, sensitive, and easy to adapt and can function in a wide range of temperatures from 5 to 65 °C.

SUBMITTER: Shen F 

PROVIDER: S-EPMC7658945 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Short DNA Oligonucleotide as a Ag<sup>+</sup> Binding Detector.

Shen Fusheng F   Mao Song S   Mathivanan Johnsi J   Wu Ying Y   Chandrasekaran Arun Richard AR   Liu Hehua H   Gan Jianhua J   Sheng Jia J  

ACS omega 20201028 44


Ag<sup>+</sup> has been known to mediate several natural metallo-base pairs. Based on the unique structural information of a short 8-mer DNA strand (5'-GCACGCGC-3') induced by Ag<sup>+</sup>, we constructed several fluorescent DNA beacons for the detection of Ag<sup>+</sup> according to the increase in the fluorescence emission on Ag<sup>+</sup> binding. This Ag<sup>+</sup> detection assay is quick, sensitive, and easy to adapt and can function in a wide range of temperatures from 5 to 65 °C. ...[more]

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