Water-Gated Proton Transfer Dynamics in Respiratory Complex I.

ABSTRACT: The respiratory complex I transduces redox energy into an electrochemical proton gradient in aerobic respiratory chains, powering energy-requiring processes in the cell. However, despite recently resolved molecular structures, the mechanism of this gigantic enzyme remains poorly understood. By combining large-scale quantum and classical simulations with site-directed mutagenesis and biophysical experiments, we show here how the conformational state of buried ion-pairs and water molecules control the protonation dynamics in the membrane domain of complex I and establish evolutionary conserved long-range coupling elements. We suggest that an electrostatic wave propagates in forward and reverse directions across the 200 Å long membrane domain during enzyme turnover, without significant dissip

SUBMITTER: Muhlbauer ME 

PROVIDER: S-EPMC7659035 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature