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Dengue in Western Uganda: a prospective cohort of children presenting with undifferentiated febrile illness.


ABSTRACT: BACKGROUND:The spatial distribution and burden of dengue in sub-Saharan Africa remains highly uncertain, despite high levels of ecological suitability. The goal of this study was to describe the epidemiology of dengue among a cohort of febrile children presenting to outpatient facilities located in areas of western Uganda with differing levels of urbanicity and malaria transmission intensity. METHODS:Eligible children were first screened for malaria using rapid diagnostic tests. Children with a negative malaria result were tested for dengue using a combination NS1/IgM/IgG rapid test (SD Bioline Dengue Duo). Confirmatory testing by RT-PCR was performed in a subset of participants. Antigen-capture ELISA was performed to estimate seroprevalence. RESULTS:Only 6 of 1416 (0.42%) children had a positive dengue rapid test, while none of the RT-PCR results were positive. ELISA testing demonstrated reactive IgG antibodies in 28 (2.2%) participants with the highest prevalence seen at the urban site in Mbarara (19 of 392, 4.9%, p 

SUBMITTER: Boyce RM 

PROVIDER: S-EPMC7661220 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Dengue in Western Uganda: a prospective cohort of children presenting with undifferentiated febrile illness.

Boyce Ross M RM   Collins Matthew M   Muhindo Rabbison R   Nakakande Regina R   Ciccone Emily J EJ   Grounds Samantha S   Espinoza Daniel D   Zhu Yerun Y   Matte Michael M   Ntaro Moses M   Nyehangane Dan D   Juliano Jonathan J JJ   Mulogo Edgar M EM  

BMC infectious diseases 20201111 1


<h4>Background</h4>The spatial distribution and burden of dengue in sub-Saharan Africa remains highly uncertain, despite high levels of ecological suitability. The goal of this study was to describe the epidemiology of dengue among a cohort of febrile children presenting to outpatient facilities located in areas of western Uganda with differing levels of urbanicity and malaria transmission intensity.<h4>Methods</h4>Eligible children were first screened for malaria using rapid diagnostic tests. C  ...[more]

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