Distribution of Protein Precipitation Capacity within Variable Proanthocyanidin Fingerprints.

ABSTRACT: Proanthocyanidins (PAs) are highly bioactive plant specialized metabolites. One of their most characteristic features is their ability to precipitate proteins. In this study, eleven plant species were used to study the structure-activity patterns between PAs and their protein precipitation capacity (PPC) with bovine serum albumin. To obtain a comprehensive selection of PAs with highly variable procyanidin to prodelphinidin ratios and mean degree of polymerizations, nearly 350 subfractions were produced from the eleven plant species by semi-preparative liquid chromatography. Their PA composition was defined by tandem mass spectrometry and high-resolution mass spectrometry, and their PPC was measured with a turbidimetry-based well-plate reader assay. The distribution of the PPC within plant

SUBMITTER: Leppa MM 

PROVIDER: S-EPMC7663390 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature