Laser scanning reflection-matrix microscopy for aberration-free imaging through intact mouse skull.

ABSTRACT: A mouse skull is a barrier for high-resolution optical imaging because its thick and inhomogeneous internal structures induce complex aberrations varying drastically from position to position. Invasive procedures creating either thinned-skull or open-skull windows are often required for the microscopic imaging of brain tissues underneath. Here, we propose a label-free imaging modality termed laser scanning reflection-matrix microscopy for recording the amplitude and phase maps of reflected waves at non-confocal points as well as confocal points. The proposed method enables us to find and computationally correct up to 10,000 angular modes of aberrations varying at every 10 × 10 µm² patch in the sample plane. We realized reflectance imaging of myelinated axons in vivo underneath a

SUBMITTER: Yoon S 

PROVIDER: S-EPMC7665219 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature