A DNA methylation state transition model reveals the programmed epigenetic heterogeneity in human pre-implantation embryos.

ABSTRACT:

Background

During mammalian early embryogenesis, expression and epigenetic heterogeneity emerge before the first cell fate determination, but the programs causing such determinate heterogeneity are largely unexplored.

Results

Here, we present MethylTransition, a novel DNA methylation state transition model, for characterizing methylation changes during one or a few cell cycles at single-cell resolution. MethylTransition involves the creation of a transition matrix comprising three parameters that represent the probabilities of DNA methylation-modifying activities in order to link the methylation states before and after a cell cycle. We apply MethylTransition to single-cell DNA methylome data from human pre-implantation embryogenesis and elucidate that the DNA methylation het

SUBMITTER: Zhao C 

PROVIDER: S-EPMC7667739 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature