A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities.
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ABSTRACT:
SUBMITTER: Shaffer JP
PROVIDER: S-EPMC7668742 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
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A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities.
Shaffer Justin P JP Marotz Clarisse C Belda-Ferre Pedro P Martino Cameron C Wandro Stephen S Estaki Mehrbod M Salido Rodolfo A RA Carpenter Carolina S CS Zaramela Livia S LS Minich Jeremiah J JJ Bryant MacKenzie M Sanders Karenina K Fraraccio Serena S Ackermann Gail G Humphrey Gregory G Swafford Austin D AD Miller-Montgomery Sandrine S Knight Rob R
bioRxiv : the preprint server for biology 20201114
One goal among microbial ecology researchers is to capture the maximum amount of information from all organisms in a sample. The recent COVID-19 pandemic, caused by the RNA virus SARS-CoV-2, has highlighted a gap in traditional DNA-based protocols, including the high-throughput methods we previously established as field standards. To enable simultaneous SARS-CoV-2 and microbial community profiling, we compare the relative performance of two total nucleic acid extraction protocols and our previou ...[more]
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