Ontology highlight
ABSTRACT:
SUBMITTER: Qi X
PROVIDER: S-EPMC7669734 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Qi Xiaofeng X Friedberg Lucas L De Bose-Boyd Ryan R Long Tao T Li Xiaochun X
Nature chemical biology 20200914 12
Smoothened (SMO), a class Frizzled G protein-coupled receptor (class F GPCR), transduces the Hedgehog signal across the cell membrane. Sterols can bind to its extracellular cysteine-rich domain (CRD) and to several sites in the seven transmembrane helices (7-TMs) of SMO. However, the mechanism by which sterols regulate SMO via multiple sites is unknown. Here we determined the structures of SMO-G<sub>i</sub> complexes bound to the synthetic SMO agonist (SAG) and to 24(S),25-epoxycholesterol (24(S ...[more]