Unknown

Dataset Information

0

High-Precision Protein-Tracking With Interferometric Scattering Microscopy.


ABSTRACT: The mobility of proteins and lipids within the cell, sculpted oftentimes by the organization of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single particle tracking has proven to be a vital tool to study the mobility of individual molecules and unravel details of their behavior. Interferometric scattering (iSCAT) microscopy is an emerging technique well-suited for visualizing the diffusion of gold nanoparticle-labeled membrane proteins to a spatial and temporal resolution beyond the means of traditional fluorescent labels. We discuss the applicability of interferometric single particle tracking (iSPT) microscopy to investigate the minutia in the motion of a protein through measurements visualizing the mobility of the epidermal growth factor receptor in various biological scenarios on the live cell.

SUBMITTER: Taylor RW 

PROVIDER: S-EPMC7669747 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

High-Precision Protein-Tracking With Interferometric Scattering Microscopy.

Taylor Richard W RW   Holler Cornelia C   Mahmoodabadi Reza Gholami RG   Küppers Michelle M   Dastjerdi Houman Mirzaalian HM   Zaburdaev Vasily V   Schambony Alexandra A   Sandoghdar Vahid V  

Frontiers in cell and developmental biology 20201103


The mobility of proteins and lipids within the cell, sculpted oftentimes by the organization of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single particle tracking has proven to be a vital tool to study the mobility of individual molecules and unravel details of their behavior. Interferometric scattering (iSCAT) microscopy is an emerging technique well-suited for visualizing the diffusion of gold nanopart  ...[more]

Similar Datasets

| S-EPMC7979857 | biostudies-literature
| S-EPMC4806212 | biostudies-literature
| S-EPMC5914294 | biostudies-literature
| S-EPMC6026387 | biostudies-literature
| S-EPMC10870763 | biostudies-literature
| S-EPMC9661846 | biostudies-literature
| S-EPMC5323080 | biostudies-literature
| S-EPMC7175421 | biostudies-literature
| S-EPMC7513470 | biostudies-literature
| S-EPMC4391024 | biostudies-literature