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RNA sequencing as an alternative tool for detecting measurable residual disease in core-binding factor acute myeloid leukemia.

ABSTRACT: DNA sequencing-based measurable residual disease (MRD) detection has shown to be clinically relevant in AML. However, the same methodology cannot be applied to fusion gene-driven subtypes of AML such as core-binding factor AML (CBF-AML). Here in this study, we evaluated the effectiveness of using DNA and RNA sequencing in MRD detection and in tracking clonal dynamics in CBF-AML. Using RNA-seq, we were able to quantify expression levels of RUNX1-RUNX1T1 and CBFB-MYH11 at diagnosis and their levels of reduction during remission (P?

SUBMITTER: Kim T

PROVIDER: S-EPMC7674449 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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RNA sequencing as an alternative tool for detecting measurable residual disease in core-binding factor acute myeloid leukemia.

Kim TaeHyung T Moon Joon Ho JH Ahn Jae-Sook JS Ahn Seo-Yeon SY Jung Sung-Hoon SH Yang Deok-Hwan DH Lee Je-Jung JJ Shin Myung-Geun MG Choi Seung Hyun SH Lee Ja-Yeon JY Tyndel Marc S MS Lee Hui Young HY Kim Kyoung Ha KH Cai Yu Y Lee Yoo Jin YJ Sohn Sang Kyun SK Min Yoo Hong YH Cheong June-Won JW Kim Hyeoung-Joon HJ Zhang Zhaolei Z Kim Dennis Dong Hwan DDH

Scientific reports 20201118 1

DNA sequencing-based measurable residual disease (MRD) detection has shown to be clinically relevant in AML. However, the same methodology cannot be applied to fusion gene-driven subtypes of AML such as core-binding factor AML (CBF-AML). Here in this study, we evaluated the effectiveness of using DNA and RNA sequencing in MRD detection and in tracking clonal dynamics in CBF-AML. Using RNA-seq, we were able to quantify expression levels of RUNX1-RUNX1T1 and CBFB-MYH11 at diagnosis and their level ...[more]

PMID: 33208771

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