Dataset Information

An Arabidopsis Cytokinin-Modifying Glycosyltransferase UGT76C2 Improves Drought and Salt Tolerance in Rice.

ABSTRACT: Drought and salt stresses are common environmental threats that negatively affect rice development and yield. Here we report that the overexpression of AtUGT76C2, a cytokinin glycosyltransferase, in rice modulates cytokinin homeostasis and confers the plants an eminent property in drought and salt tolerance. The transgenic plants exhibit sensitivity to salt and drought stress as well as abscisic acid during the germination stage and the postgermination stage while showing enhanced tolerance to drought and salinity at the young seedling stage and the mature stage. The overexpression of UGT76C2 decreases the endogenous cytokinin level and enhances root growth, which greatly contributes to stress adaptation. In addition, the transgenic plants also show enhanced ROS scavenging activity, reduced ion leakage under salt stress, smaller stomatal opening, and more proline and soluble sugar accumulation, which demonstrate that UGT76C2 acts as an important player in abiotic stress response in rice. To explore the molecular mechanism of UGT76C2 in response to stress adaptation, the expressions of eight stress-responsive genes including OsSOS1, OsPIP2.1, OsDREB2A, OsCOIN, OsABF2, OsRAB16, OsP5CR, and OsP5CS1 were detected, which showed notable upregulation in UGT76C2 overexpression plants under salt and drought stresses. Our results reveal that the ectopic expression of AtUGT76C2 confers the transgenic rice many traits in improving drought and salt stress tolerance in both developmental and physiological levels. It is believed that AtUGT76C2 could be a promising candidate gene for cultivating saline- and drought-tolerant rice.

SUBMITTER: Li Y

PROVIDER: S-EPMC7674613 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

An <i>Arabidopsis</i> Cytokinin-Modifying Glycosyltransferase UGT76C2 Improves Drought and Salt Tolerance in Rice.

Li Yanjie Y Liu Fangfei F Li Pan P Wang Ting T Zheng Chengchao C Hou Bingkai B

Frontiers in plant science 20201105

Drought and salt stresses are common environmental threats that negatively affect rice development and yield. Here we report that the overexpression of <i>AtUGT76C2</i>, a cytokinin glycosyltransferase, in rice modulates cytokinin homeostasis and confers the plants an eminent property in drought and salt tolerance. The transgenic plants exhibit sensitivity to salt and drought stress as well as abscisic acid during the germination stage and the postgermination stage while showing enhanced toleran ...[more]

PMID: 33224159

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Project description:The NAC transcription factors play critical roles in regulating stress responses in plants. However, the functions for many of the NAC family members in rice are yet to be identified. In the present study, a novel stress-responsive rice NAC gene, ONAC022, was identified. Expression of ONAC022 was induced by drought, high salinity, and abscisic acid (ABA). The ONAC022 protein was found to bind specifically to a canonical NAC recognition cis-element sequence and showed transactivation activity at its C-terminus in yeast. The ONAC022 protein was localized to nucleus when transiently expressed in Nicotiana benthamiana. Three independent transgenic rice lines with overexpression of ONAC022 were generated and used to explore the function of ONAC022 in drought and salt stress tolerance. Under drought stress condition in greenhouse, soil-grown ONAC022-overexpressing (N22oe) transgenic rice plants showed an increased drought tolerance, leading to higher survival ratios and better growth than wild-type (WT) plants. When grown hydroponically in Hogland solution supplemented with 150 mM NaCl, the N22oe plants displayed an enhanced salt tolerance and accumulated less Na(+) in roots and shoots as compared to WT plants. Under drought stress condition, the N22oe plants exhibited decreased rates of water loss and transpiration, reduced percentage of open stomata and increased contents of proline and soluble sugars. However, the N22oe lines showed increased sensitivity to exogenous ABA at seed germination and seedling growth stages but contained higher level of endogenous ABA. Expression of some ABA biosynthetic genes (OsNCEDs and OsPSY), signaling and regulatory genes (OsPP2C02, OsPP2C49, OsPP2C68, OsbZIP23, OsAP37, OsDREB2a, and OsMYB2), and late stress-responsive genes (OsRAB21, OsLEA3, and OsP5CS1) was upregulated in N22oe plants. Our data demonstrate that ONAC022 functions as a stress-responsive NAC with transcriptional activator activity and plays a positive role in drought and salt stress tolerance through modulating an ABA-mediated pathway.

Dataset Information

An Arabidopsis Cytokinin-Modifying Glycosyltransferase UGT76C2 Improves Drought and Salt Tolerance in Rice.

Publications

An <i>Arabidopsis</i> Cytokinin-Modifying Glycosyltransferase UGT76C2 Improves Drought and Salt Tolerance in Rice.

Similar Datasets

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