Dataset Information

Transcriptome-Wide m⁶A Methylation in Skin Lesions From Patients With Psoriasis Vulgaris.

ABSTRACT: N⁶-methyladenosine (m⁶A) methylation, as the most prevalent internal RNA modification, has been revealed to play critical roles in various biological functions. In this study, we performed m⁶A transcriptome-wide profiling in three kinds of skin tissue: involved psoriatic skin (PP), uninvolved psoriatic skin (PN), and healthy control skin samples (NN). The findings revealed that transcripts of PP contained the fewest m⁶A peaks and lowest m⁶A peak density. The greatest differences of m⁶A methylation were observed in the PP vs. NN and PP vs. PN comparisons. Intriguingly, in these comparisons, hypermethylated m⁶A was mainly enriched within the CDSs and 3'UTRs, while hypomethylated m⁶A was not only enriched within CDSs and 3'UTRs, but also within 5'UTRs. GO and KEGG pathway analyses indicated that hypermethylated transcripts in PP were particularly associated with response-associated terms, cytokine production, and olfactory transduction. Meanwhile, hypomethylated transcripts in PP were mainly associated with development-related processes and the Wnt signaling pathway. In addition, we discovered that 19.3-48.4% of the differentially expressed transcripts in psoriasis vulgaris were modified by m⁶A, and that transcripts with lower expression were more preferentially modified by m⁶A. Moreover, upregulation of gene expression was often accompanied by upregulation of m⁶A methylation, suggesting a regulatory role of m⁶A in psoriasis vulgaris gene expression.

SUBMITTER: Wang YN

PROVIDER: S-EPMC7674922 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Transcriptome-Wide m6A Methylation in Skin Lesions From Patients With Psoriasis Vulgaris.

Wang Ya-Nan YN Jin Hong-Zhong HZ

Frontiers in cell and developmental biology 20201105

N6-methyladenosine (m6A) methylation, as the most prevalent internal RNA modification, has been revealed to play critical roles in various biological functions. In this study, we performed m6A transcriptome-wide profiling in three kinds of skin tissue: involved psoriatic skin (PP), uninvolved psoriatic skin (PN), and healthy control skin samples (NN). The findings revealed that transcripts of PP contained the fewest m6A peaks and lowest m6A ...[more]

PMID: 33251217

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Project description:BackgroundCucumber green mottle mosaic virus (CGMMV) causes substantial global losses in cucurbit crops, especially watermelon. N6-methyladenosine (m6A) methylation in RNA is one of the most important post-transcriptional modification mechanisms in eukaryotes. It has been shown to have important regulatory functions in some model plants, but there has been no research regarding m6A modifications in watermelon.ResultsWe measured the global m6A level in resistant watermelon after CGMMV infection using a colorimetric method. And the results found that the global m6A level significantly decreased in resistant watermelon after CGMMV infection. Specifically, m6A libraries were constructed for the resistant watermelon leaves collected 48 h after CGMMV infection and the whole-genome m6A-seq were carried out. Numerous m6A modified peaks were identified from CGMMV-infected and control (uninfected) samples. The modification distributions and motifs of these m6A peaks were highly conserved in watermelon transcripts but the modification was more abundant than in other reported crop plants. In early response to CGMMV infection, 422 differentially methylated genes (DMGs) were identified, most of which were hypomethylated, and probably associated with the increased expression of watermelon m6A demethylase gene ClALKBH4B. Gene Ontology (GO) analysis indicated quite a few DMGs were involved in RNA biology and stress responsive pathways. Combined with RNA-seq analysis, there was generally a negative correlation between m6A RNA methylation and transcript level in the watermelon transcriptome. Both the m6A methylation and transcript levels of 59 modified genes significantly changed in response to CGMMV infection and some were involved in plant immunity.ConclusionsOur study represents the first comprehensive characterization of m6A patterns in the watermelon transcriptome and helps to clarify the roles and regulatory mechanisms of m6A modification in watermelon in early responses to CGMMV.

Dataset Information

Transcriptome-Wide m⁶A Methylation in Skin Lesions From Patients With Psoriasis Vulgaris.

Publications

Transcriptome-Wide m<sup>6</sup>A Methylation in Skin Lesions From Patients With Psoriasis Vulgaris.

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