Dataset Information

Laser Desorption Ionization Time-of-Flight Mass Spectrometry of Silver-Doped (GeS2)50(Sb2S3)50 Chalcogenide Glasses.

ABSTRACT: Mass spectra of (GeS2)50(Sb2S3)50 glass and Ag-doped glasses [5% Ag (GeS2)50(Sb2S3)50 and 15% Ag (GeS2)50(Sb2S3)50] obtained using laser desorption ionization (LDI) time-of-flight coupled with quadrupole ion trap mass spectrometry were studied. The analysis of the mass spectra indicated the formation of Ag a Ge b Sb c S d clusters. In addition to the SbS d + (d = 1 and 2), Sb2S d + (d = 1-3), Sb3S d + (d = 1-5), Sb4S d + (d = 3 and 4), Sb5S2 +, and Sb c + (c = 3 and 5) clusters, various clusters containing Ag, such as Ag a + (a = 1 and 2), AgGeS+, AgSb c + (c = 1, 2, and 4), AgSbS+, AgSb2S d + (d = 1-5), AgSb3S3 +, AgSb4S4 +, Ag2Sb3S d + (d = 4 and 5), Ag4Sb2S3 +, and Ag5SbS3 +, were generated. Moreover, in spite of the five-ninth purity of all glass components, several hydrogenated clusters (SbS3H8 +, Sb4S2H+, Ag2H11 +, Ag2Sb3H4 +, Ag3Sb2H4 +, Ag4Sb2H2 +, and Ag4S3H8 +) and some low-intensity oxidized clusters, such as Sb3O+ and Sb3O5 +, were also detected. When applying LDI on (GeS2)50(Sb2S3)50 glass, no Ge-containing clusters were detected in the positive ion mode, and just one Ge-containing cluster was observed after doping the glass with Ag. Hydrogen plays an important role in the glasses studied. The knowledge gained concerning cluster stoichiometry contributes to the elucidation of the structure of Ag-doped Ge-Sb-S chalcogenide glasses. It should be noted that some of the clusters were considered to be structural fragments. Furthermore, mass spectrometry was complemented with Raman spectroscopy.

SUBMITTER: Huang F

PROVIDER: S-EPMC7675535 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Laser Desorption Ionization Time-of-Flight Mass Spectrometry of Silver-Doped (GeS2)50(Sb2S3)50 Chalcogenide Glasses.

Huang Fei F Wágner Tomáš T Frumarová Božena B Fraenkl Max M Koštál Petr P Havel Josef J

ACS omega 20201109 45

Mass spectra of (GeS2)50(Sb2S3)50 glass and Ag-doped glasses [5% Ag (GeS2)50(Sb2S3)50 and 15% Ag (GeS2)50(Sb2S3)50] obtained using laser desorption ionization (LDI) time-of-flight coupled with quadrupole ion trap mass spectrometry were studied. The analysis of the mass spectra indicated the formation of Ag a Ge <sub ...[more]

PMID: 33225126

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Project description:Streptococcus suis, particularly S. suis serotype 2 (SS2), is an important zoonotic pathogen causing meningitis in humans worldwide. Although the proper classification of the causative and pathogenic serotype is salutary for the clinical diagnosis, cross-reactions leading to the indistinguishability of serotypes by the current serotyping methods are significant limitations. In the present study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of extracted peptides was developed to improve the classification of serotype of S. suis. The peptide mass fingerprint (PMFs) database of S. suis was generated from the whole-cell peptides of 32 reference strains of S. suis isolates obtained from pigs. Thirty-two human S. suis isolates from clinical cases in Thailand were used to validate this alternative serotyping method in direct comparison to the multiplex (m)PCR approach. All reference strains, representing 32 serotypes of S. suis, exhibited their individual PMFs patterns, thus allowing differentiation from one another. Highly pathogenic SS2 and SS14 were clearly differentiated from the otherwise serologically closely related SS1/2 and SS1, respectively. The developed MALDI-TOF-MS serotyping method correctly classified the serotype in 68.8% (22/32) of the same serotype isolates generated from the PMFs database; while the validity for the clinical human isolates was 62.5% (20/32). The agreement between the MALDI-TOF-MS and mPCR serotyping was moderate with a Kappa score of 0.522, considering that mPCR could correctly serotype up to 75%. The present study demonstrated that PMFs from the developed MALDI-TOF-MS-based method could successfully discriminate the previously indistinguishable highly pathogenic SS2 and SS14 from SS1/2 and SS1, respectively. Moreover, this serotyping method distinguished pathogenic SS6, and so is an alternative approach of choice to rapidly and reliably serotype clinically pathogenic S. suis isolates.

Dataset Information

Laser Desorption Ionization Time-of-Flight Mass Spectrometry of Silver-Doped (GeS2)50(Sb2S3)50 Chalcogenide Glasses.

Publications

Laser Desorption Ionization Time-of-Flight Mass Spectrometry of Silver-Doped (GeS<sub>2</sub>)<sub>50</sub>(Sb<sub>2</sub>S<sub>3</sub>)<sub>50</sub> Chalcogenide Glasses.

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